法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (4): 285-286.DOI: 10.3969/j.issn.1004-5619.2010.04.014

• 技术与应用 • 上一篇    下一篇

PowerPlex 16 HS系统直接扩增法检测常见检材的DNA

周  密，唐  涛，韦  帆，张韩秋，李欣欣，丁仁杰，周  进，王成权   

  1. （芜湖市公安局 刑警支队，安徽 芜湖 241000）
  • 发布日期:2010-08-25 出版日期:2010-08-28
  • 作者简介:周密（1982—），男，安徽芜湖人，硕士，主要从事法医物证学研究；E－mail：protease168@yahoo.com.cn

Application of Direct Amplification with PowerPlex 16 HS System for Detection of DNA in Conventional Evidence Materials

ZHOU MI, TANG TAO, WEI FAN, ZHANG HAN-QIU, LI XIN-XIN, DING REN-JIE, ZHOU JIN, WANG CHENG-QUAN   

  1. （Criminal Police Branch, Wuhu Public Security Bureau, Wuhu 241000, China）
  • Online:2010-08-25 Published:2010-08-28

摘要： 目的 研究PowerPlex 16 HS系统直接扩增法对血样、口腔拭子和烟蒂3种常见检材的有效性。 方法 采用PowerPlex 16 HS系统对11份新鲜血样、10份口腔拭子和10份烟蒂样本进行直接扩增检测，对所得数据进行统计分析。 结果 新鲜血样和口腔拭子的完整遗传图谱检测成功率为100%，烟蒂样本的完整遗传图谱检测成功率为90%。 结论 PowerPlex 16 HS系统直接扩增法是一种适用于血样、口腔拭子和烟蒂3种常见检材的有效方法。

关键词: 法医遗传学, 核酸扩增技术, PowerPlex 16 HS系统

Abstract: Objective To study the effectiveness of direct amplification with PowerPlex 16 HS system for DNA detection in three conventional materials: fresh blood, oral swab, and cigarette butt. Methods The genetic data of 11 samples of fresh blood, 10 samples of oral swab and 10 samples of cigarette butts were analyzed with PowerPlex 16 HS kit. The result was statistically analyzed. Results Success rate of whole genetic map test in fresh blood and oral swab samples was 100%. Success rate in cigarette butt samples was 90%. Conclusion Direct amplification with PowerPlex 16 HS system is effective method to three types of conventional evidence material including fresh blood, oral swab and cigarette butt.

Key words: forensic genetics, nucleic acid amplification techniques, PowerPlex 16 HS system

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