法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (2): 87-90.DOI: 10.3969/j.issn.1004-5619.2011.02.002

• 论著 • 上一篇    下一篇

血管细胞黏附分子-1在过敏性休克大鼠器官中的表达

李晓洁，郭相杰，高彩荣   

  1. （山西医科大学 法医学院，山西 太原 030001）
  • 发布日期:2011-04-25 出版日期:2011-04-28
  • 通讯作者: 通信作者：高彩荣，女，博士，教授，博士研究生导师，主要从事法医病理学的教学、研究和检案工作；E-mail：gaocairong5175@163.com
  • 作者简介:李晓洁（1984—），女，山西万荣人，硕士研究生，主要从事法医病理学研究

The Expression of VCAM-1 in Organs of Rats Died of Anaphylactic Shock

LI XIAO-JIE, GUO XIANG-JIE, GAO CAI-RONG   

  1. （School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China）
  • Online:2011-04-25 Published:2011-04-28

被引次数

11

摘要： 目的 探讨血管细胞黏附分子-1（vascular cell adhesion molecule-1，VCAM-1）在过敏性休克大鼠器官中的作用及其表达规律。 方法 制造大鼠过敏性休克模型，采用免疫组化SABC法分别检测：（1）过敏性休克大鼠肺、心、脑、肾、肝、脾、胃、肠中VCAM-1的表达；（2）过敏性休克不同时间（休克10、30 min和死亡时）大鼠肺内VCAM-1的表达；（3）在诱导大鼠过敏性休克后用抗VCAM-1干预时大鼠肺内VCAM-1的表达。 结果 在过敏性休克大鼠死亡时，肺内VCAM-1的表达与对照组相比显著增多，而且随着休克时间延长，VCAM-1表达有增多趋势，休克后抗VCAM-1处理组肺内的VCAM-1表达相对减少。 结论 （1）VCAM-1在过敏性休克大鼠各器官中的表达呈现显著差异性，肺内变化最明显，随着过敏性休克的进展有上升趋势。（2）过敏性休克发生后，抗VCAM-1能抑制大鼠肺内VCAM-1的表达。

关键词: 法医病理学, 过敏反应, 血管细胞黏附分子-1, 大鼠

Abstract: Objective To investigate the effect and expression of the vascular cell adhesion molecule-1（VCAM-1） in organs of rats died of anaphylactic shock. Methods The models of anaphylactic shock in rats were made and the immunohistochemisty of SABC was used to detect as follows： （1） The expression of VCAM-1 in rat lung, heart, brain, kidney, liver, spleen, stomach and intestine. （2） VCAM-1 levels in lungs at 10 min, 30 min after the allergic shock, and the time of death. （3） VCAM-1 levels in lungs of rats after the intervention of anti-VCAM-1. Results After the death, the expression VCAM-1 in lungs increased significantly relative to the control group and followed the extension of shock. In the rats which were injected with the anti-VCAM-1, the expression of VCAM-1 in lungs reduced. Conclusion （1） The expression of VCAM-1 shows difference in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs and would increase followed the extension of anaphylactic shock. （2） After the anaphylactic shock, anti-VCAM-1 can inhibit the expression of VCAM-1 in rat lung.

Key words: forensic pathology, anaphylaxis, vascular cell adhesion molecule-1, rats

中图分类号: