法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (3): 193-195.DOI: 10.3969/j.issn.1004-5619.2011.03.010

• 论著 • 上一篇    下一篇

微波消解ICP-MS法检测生物检材中汞元素

马  栋1，2，张  丹2，3，卓先义2，刘  伟2，沈保华2，沈  敏2   

  1. （1. 复旦大学 上海医学院法医学系，上海 200032； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063； 3. 苏州大学 医学部法医学系，江苏 苏州 215123）
  • 发布日期:2011-06-25 出版日期:2011-06-28
  • 通讯作者: 通信作者：沈敏，女，研究员，博士研究生导师，主要从事法医毒物分析；E-mail：shenm@ssfjd.cn
  • 作者简介:马栋（1978—），男，甘肃张家川人，助理研究员，主要从事法医毒物分析研究；E-mail：madong@ssfjd.cn

Determination of Hg in Biological Samples by Inductively Coupled Plasma Mass Spectrometry

MA DONG1,2, ZHANG DAN2,3, ZHUO XIAN-YI2, LIU WEI2, SHEN BAO-HUA2, SHEN MIN2   

  1. （1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 3. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China）
  • Online:2011-06-25 Published:2011-06-28

被引次数

30

摘要： 目的 建立生物检材中汞的电感耦合等离子体质谱分析方法。 方法 采用微波消解法处理样品，以铟（115In）作内标，用电感耦合等离子体质谱仪对血液、尿液和头发中的汞含量进行分析。选择金与汞形成金汞齐，对金消除汞记忆效应的能力进行考察。 结果 方法检出限为0.01 µg/L，准确度为97.0%～107.1％。检测中添加金质量浓度在10 µg/L时，可消除检测中汞的记忆效应。 结论 建立的方法准确、快速、灵敏度高，适用于汞中毒案件的检测以及临床中汞中毒病人的诊断和监测。

关键词: 法医毒理学, 汞, 电感耦合等离子体质谱法, 微波消解

Abstract: Objective To establish an inductively coupled plasma mass spectrometry（ICP-MS） method for determination of Hg in biological samples. Methods The samples were digested with microwave digestion instrument. ICP-MS was applied to detect Hg in blood, urine and hair specimens by using 115In as an internal marker. The ability of gold to eliminate the memory effect of mercury was investigated with the gold amalgamate produced by gold and mercury. Results The limits of detection were in the 0.01 µg/L, and the accuracy of the method ranged from 97.0% to 107.1%. The concentration of gold was 10 µg/L and the memory effect of mercury was resolved. Conclusion The method is accurate, rapid, sensitive and suitable for the cases of mercury poisoning and the clinical diagnosis and monitoring for patients with mercury poisoning.

Key words: forensic toxicology, mercury, inductively coupled plasma mass spectrometry, microwave digestion

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