法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (4): 271-273.DOI: 10.3969/j.issn.1004-5619.2011.04.008

• 论著 • 上一篇    下一篇

GC-MS同时测定血液中苯海索、氯丙嗪和氯氮平

郑水庆，王  威，梁  晨，汪  蓉，龚飞君，吴忠平，陈永生，张玉荣，张润生   

  1. （上海市公安局物证鉴定中心 上海市现场物证重点实验室，上海 200083）
  • 发布日期:2011-08-25 出版日期:2011-08-28
  • 通讯作者: 通信作者：张润生，男，主任法医师，主要从事毒物和毒品的检测工作；E-mail：zhangrs@sh163.net
  • 作者简介:郑水庆（1973—），男，浙江衢州人，博士，工程师，主要从事毒物、毒品及有毒植物的检测工作

Simultaneous Determination of Trihexyphenidyl, Chlorpromazine and Clozapine in Blood by GC-MS

ZHENG SHUI-QING, WANG WEI, LIANG CHEN, WANG RONG, GONG FEI-JUN, WU ZHONG-PING, CHEN YONG-SHENG, ZHANG YU-RONG, ZHANG RUN-SHENG   

  1. （Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China）
  • Online:2011-08-25 Published:2011-08-28

摘要： 目的 建立GC-MS同时测定血液中苯海索、氯丙嗪和氯氮平的方法。 方法 血液中加入内标SKF525A，在pH>10条件下用V（苯）﹕V（乙酸乙酯）=1﹕1提取，用GC-MS全扫描法进行定性检测；以地西泮-d5为内标，样品在pH>10条件下用V（苯）∶V（乙酸乙酯）=1∶1提取，用GC-MS选择离子监测方法进行定量检测。 结果 苯海索、氯丙嗪和氯氮平在20～10 000 ng/mL范围内呈线性关系，最小检测限分别为0.3、0.3和0.7 ng/mL（信噪比≥3），方法回收率为79.9%~85.5%，日内、日间精密度均小于5.1%。 结论 本方法可同时分析血液中苯海索、氯丙嗪和氯氮平，方法灵敏度高、快速、操作简便，适用于苯海索、氯丙嗪和氯氮平的血药浓度监测和急性中毒案件的检测。

关键词: 法医毒理学, 气相色谱-质谱法, 苯海索, 氯丙嗪, 氯氮平, 血液

Abstract: Objective To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS. Methods The specimens were alkalized（pH>10） and extracted with V（benzene）∶V（ethyl acetate）=1∶1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized（pH>10） and extracted with V（benzene）∶V（ethyl acetate）=1∶1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5. Results The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL（S/N≥3） respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%. Conclusion The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.

Key words: forensic toxicology, GC-MS, trihexyphenidyl, chlorpromazine, clozapine, blood

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