法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (6): 441-443.DOI: 10.3969/j.issn.1004-5619.2011.06.011

• 技术与应用 • 上一篇    下一篇

二次消化结合磁珠法提取腐败组织DNA

杨  电，刘  超，刘  宏   

  1. （广州市刑事科学技术研究所，广东 广州 510030）
  • 发布日期:2011-12-25 出版日期:2011-12-28
  • 作者简介:杨电（1969—），女，广东大埔人，硕士，副主任法医师，主要从事法医DNA检验与研究工作；Tel：020-83118387
  • 基金资助:

    公安部应用创新计划资助项目（2005YYCX57136）；广东省重点实验室建设项目（2010A060801001）

DNA Extraction from Decomposed Tissue by Double-digest and Magnetic Beads Methods

YANG DIAN, LIU CHAO, LIU HONG   

  1. （Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China）
  • Online:2011-12-25 Published:2011-12-28

被引次数

6

摘要： 目的 研究二次消化结合磁珠法对3种腐败组织DNA的提取效果。 方法 采用二次消化结合磁珠法对用常规磁珠法提取DNA失败的91例高度腐败尸体的软骨、指（趾）甲、关节囊组织提取DNA，QuantifilerTM试剂盒进行DNA定量，用SinofilerTM或MinifilerTM试剂盒进行STR检测分型。 结果 91例高度腐败的软骨、指（趾）甲、关节囊组织提取的DNA质量浓度在0~0.225 ng/μL，其中62例DNA质量浓度在0.020 ng/μL以上的检材成功获得9个以上STR基因座分型图谱，检验成功率为68.13%。 结论 二次消化结合磁珠法可提高3种腐败组织DNA的STR检验成功率。

关键词: 法医遗传学, 软骨, 指（趾）甲, 关节囊, 腐败组织, 磁珠法, 二次消化

Abstract: Objective To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues. Methods DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with QuantifilerTM kit, followed by amplification with SinofilerTM kit or MinifilerTM kit. Results DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/μL. Sixty-two samples whose DNA concentration were more than 0.020 ng/μL had obtained 9 or more STR loci successfully. The detection rate was 68.13%. Conclusion The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.

Key words: forensic genetics, cartilage, nails, joint capsule, decomposed tissue, magnetic beads methods, twice-digest

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