法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (3): 164-167.DOI: 10.3969/j.issn.1004-5619.2013.03.002

• 论著 • 上一篇    下一篇

Spry1和MAPK蛋白在病毒性心肌炎心肌组织中的表达

许弘飞1，2，孟  林3，姚  健3，谷振勇4，刘国庆4，沈忆文5，赵子琴5   

  1. （1. 苏州大学医学部基础医学与生物科学学院法医学系，江苏 苏州 215123； 2. 南通通大化学物安全性评价中心，江苏 南通 226001； 3. 南通市公安局刑警支队，江苏 南通 226001； 4. 南通大学医学院法医学系，江苏 南通 226001； 5. 复旦大学上海医学院法医学系，上海 200032）
  • 发布日期:2013-06-25 出版日期:2013-06-28
  • 通讯作者: 赵子琴，女，教授，博士研究生导师，主要从事法医病理学研究；E-mail：zqzhao@shmu.edu.cn
  • 作者简介:许弘飞（1980—），男，安徽芜湖人，博士，讲师，主要从事心源性猝死的法医病理学研究；E-mail：whhongfei@sina.com
  • 基金资助:

    国家自然科学基金资助项目（81172897）；南通市自然科学基金资助项目（BK2011053）

Myocardial Expression of Spry1 and MAPK Proteins of Viral Myocarditis

XU HONG-FEI1,2, MENG LIN3, YAO JIAN3, GU ZHEN-YONG4, LIU GUO-QING4, SHEN YI-WEN5, ZHAO ZI-QIN5   

  1. （1． Department of Forensic Medicine, School of Biology and Basic Medical Sciences, Medical College of Soochow University, Suzhou 215123, China; 2. Nantong Tongda Chemicals Safety Evaluation Center, Nantong 226001, China; 3. Criminal Police Branch, Nantong Public Security Bureau, Nantong 226001, China; 4. Department of Forensic Medicine, Medical College of Nantong University, Nantong 226001, China; 5. Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China）
  • Online:2013-06-25 Published:2013-06-28

被引次数

5

摘要： 目的 通过探讨Spry1和MAPK蛋白在病毒性心肌炎（viral myocarditis，VMC）心肌组织中的表达，揭示其发病及猝死机制，并为法医学心源性猝死的鉴定提供指导。 方法 30只Balb/c雄性小鼠随机分为VMC组和对照组，分别腹腔注射柯萨奇B3病毒和Eagel’s培养液，处死小鼠取心脏，进行常规病理检查，并通过免疫组织化学、Western印迹法及real-time PCR方法检测心肌组织中Spry1蛋白及mRNA、MAPK蛋白表达变化。 结果 VMC组在光镜下可见心肌间质水肿，广泛炎症细胞浸润，心肌细胞坏死，心肌纤维形成多处灶性、大片状坏死，心肌组织中Spry1蛋白表达水平低于对照组（P<0.05），Spry1 mRNA水平略降低（P>0.05）；而MAPK蛋白表达水平在VMC组高于对照组（P<0.05）。 结论 Spry1参与的MAPK/ERK通路在病毒性心肌炎心肌纤维化过程中起着促进胶原表达的作用，从而导致心律失常、心功能减退甚至心源性猝死。

关键词: 法医病理学, 猝死, 心脏, 病毒性心肌炎, Spry蛋白, MAPK蛋白

Abstract: Objective To discuss the myocardial expression of Spry1 and MAPK proteins of viral myocarditis （VMC）, to reveal its mechanism of sudden death, and to provide guides for forensic identification of sudden cardiac death. Methods Thirty Balb/c male mice were randomly divided into VMC group and control group, inoculated intraperitoneally with Coxsackievirus B3 and Eagel’s solution, respectively．After the mice were sacrificed, the cardiac tissues of the mice were taken to proceed regular pathological examination. The changes of Spry1 protein, Spry1 mRNA and MAPK protein were detected by immunohistochemistry, Western blotting and real-time PCR. Results Under light microscope, the pathologic changes included myocardial interstitial edema, inflammatory cells infiltration, myocardial necrosis, and focal and patchy necrosis of myocardial fiber in VMC group. The expression of Spry1 protein in VMC group was lower than that in control group （P<0.05）. There was slightly decreased expression of Spry1 of the mRNA level in VMC group （P>0.05）. But the MAPK protein expression in VMC group was higher than that in control group （P<0.05）. Conclusion The pathway of MAPK/ERK involving Spry1 protein accelerates the expression of collagen, which may contribute to arrhythmia, heart failure and even sudden cardiac death.

Key words: forensic pathology, death, sudden, cardiac, vital myocarditis, Spry protein, MAPK protein

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