法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (3): 206-208.DOI: 10.3969/j.issn.1004-5619.2013.03.013

• 技术与应用 • 上一篇    下一篇

北京地区汉族人群16个非CODIS STR基因座的遗传多态性

张庆霞，杨  剑，刘雅诚，唐  晖，焦章平   

  1. （北京市公安司法鉴定中心，北京 100192）
  • 发布日期:2013-06-25 出版日期:2013-06-28
  • 作者简介:张庆霞（1978—），女，河南淮阳人，硕士，副主任法医师，主要从事法医DNA检案研究；E-mail：qingxiazhang@sina.com

Genetic Polymorphisms of 16 Non-CODIS STR Loci in Beijing Han Population

ZHANG QING-XIA, YANG JIAN, LIU YA-CHENG, TANG HUI, JIAO ZHANG-PING   

  1. （Forensic Science Service of the Beijing Public Security Bureau, Beijing 100192, China）
  • Online:2013-06-25 Published:2013-06-28

被引次数

8

摘要： 目的 调查16个非CODIS STR基因座（D6S477、D22-GATA198B05、D15S659、D8S1132、D3S3045、D17S1290、D14S608、D2S441、D18S535、D13S325、D10S1435、D11S2368、D1S1656、D7S3048、D10S1248和D19S253）在北京地区汉族人群的遗传多态性。 方法 应用GoldeneyeTM DNA身份鉴定系统18NC试剂盒，对北京地区300名汉族无关个体DNA进行PCR扩增，3130XL遗传分析仪电泳分析，GeneMapper v3.2分析等位基因片段大小。 结果 16个非CODIS STR基因座在北京地区汉族群体基因型频率分布符合Hardy-Weinberg平衡，杂合度在0.677～0.873，个体识别率在0.890～0.967，非父排除率在0.393～0.741，多态信息含量在0.706～0.853。 结论　这16个非CODIS STR基因座在北京地区汉族群体中具有较好的多态性，对群体遗传学研究和法医学应用具有重要意义。

关键词: 法医遗传学, 多态现象, 遗传, 短串联重复序列, 汉族

Abstract: Objective To investigate the genetic polymorphisms of 16 non-CODIS loci （D6S477, D22-GATA198B05, D15S659, D8S1132, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, D11S2368, D1S1656, D7S3048, D10S1248 and D19S253） in Beijing Han population. Methods The DNA of 300 unrelated individuals in Beijing Han population were PCR amplified using GoldeneyeTM DNA identification system 18NC kit, and the PCR products were analyzed by electrophoresis through 3130XL genetic analyzer. The fragment sizes of alleles were taken subsequently by GeneMapper v3.2. Results The distributions of genotype frequencies of 16 non-CODIS STR loci in Beijing Han population satisfied the Hardy-Weinberg equilibration. The population genetic parameters were obtained as followings: heterozygosity was 0.677-0.873; discrimination power, 0.890-0.967; probability of paternity exclusion, 0.393- 0.741; and polymorphism information content, 0.706-0.853. Conclusion These 16 non-CODIS STR loci show great genetic polymorphisms in Beijing Han population, and are useful for the research of population genetics and forensic application.

Key words: forensic genetics, polymorphism, genetic, short random repeats, Han nationality

中图分类号: