法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (3): 209-211.DOI: 10.3969/j.issn.1004-5619.2013.03.014

• 技术与应用 • 上一篇    下一篇

改良TRIzol法同步提取血液RNA和DNA

秦娟娟1，路志勇2，焦章平2，朱晓君2，王颖希1，唐  晖2   

  1. （1. 山西医科大学基础医学院法医学系，山西 太原 030001； 2. 北京市公安局刑侦总队，北京 100192）
  • 发布日期:2013-06-25 出版日期:2013-06-28
  • 通讯作者: 唐晖，女，主任法医师，主要从事法医DNA检验和研究；E-mail：huihuitangno1@163.com
  • 作者简介:秦娟娟（1987—），女，山西临汾人，硕士研究生，主要从事法医DNA研究；E-mail：594570279@qq.com
  • 基金资助:

    北京市科技计划资助项目（Z111100056811024）

Modified TRIzol Method for RNA and DNA Co-extraction from Blood

QIN JUAN-JUAN1, LU ZHI-YONG2, JIAO ZHANG-PING2, ZHU XIAO-JUN2, WANG YING-XI1, TANG HUI2   

  1. （1. Department of Forensic Medicine, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Criminal Investigation Team, Beijing Public Security Bureau, Beijing 100192, China）
  • Online:2013-06-25 Published:2013-06-28

被引次数

5

摘要： 目的 建立一种应用TRIzol试剂针对同一生物检材在提取总RNA的同时又能提取DNA的新方法。 方法 使用传统TRIzol法将含有总RNA的水相移除，调节中间层和有机相的pH值，乙醇沉淀DNA，运用DNA IQTM system试剂盒纯化DNA。检测DNA纯度和质量浓度，并以之为模板进行PCR-STR分型。与传统TRIzol法提取的基因组DNA进行比较。 结果 改良TRIzol法提取的基因组DNA较传统TRIzol法提取的基因组DNA纯度和质量浓度高，扩增后STR分型良好。 结论 改良TRIzol法可靠易行，能够实现同一样本同时提取RNA和DNA的目的，可以满足法庭科学个体识别和亲缘鉴定的要求。

关键词: 法医遗传学, 提取法, TRIzol法, 血液

Abstract: Objective To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent. Methods After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQTM system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method. Results The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing. Conclusion The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.

Key words: forensic genetics, extraction, TRIzol method, blood

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