法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (4): 256-258,272.DOI: 10.3969/j.issn.1004-5619.2013.04.005

• 论著 • 上一篇    下一篇

利用人体外周血痕sjTREC含量推断个体年龄

曲冬阳1，邓淑娇2，葛芸英3，陈  松3，欧雪玲1   

  1. （1. 中山大学中山医学院法医物证教研室，广东 广州 510080； 2. 广州市公安局白云区分局，广东 广州 510420； 3. 公安部物证鉴定中心 法医遗传学公安部重点实验室，北京 100038）
  • 发布日期:2013-08-25 出版日期:2013-08-28
  • 通讯作者: 葛芸英，女，硕士，副主任法医师，主要从事法医遗传学研究；E-mail：easygyy@yahoo.com.cn
  • 作者简介:曲冬阳（1988—），男，硕士研究生，主要从事法医物证学研究；E-mail：qudongyang1988@hotmail.com
  • 基金资助:

    国家自然科学基金资助项目（81001352）；中央级公益性科研院所基本科研业务费专项资金项目（2011JB007）；中央高校基本科研业务费中山大学青年教师培育计划项目（12ykpy04）

Age Estimation Using Content of sjTREC in Human Peripheral Blood

QU DONG-YANG1, DENG SHU-JIAO2, GE YUN-YING3, CHEN SONG3, OU XUE-LING1   

  1. （1. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 2. Baiyun District Sub-bureau, Guangzhou Municipal Public Security Bureau, Guangzhou 510420, China; 3. Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China）
  • Online:2013-08-25 Published:2013-08-28

被引次数

5

摘要： 目的 验证人外周血中信号结合T细胞受体删除DNA环（signal joint T-cell receptor excision DNA circle，sjTREC）水平与年龄的相关性公式，探讨其在鉴定实践中的应用价值。 方法 收集已知年龄的健康无关个体外周血痕样品30例，室温放置4周后，分别提取血痕DNA。通过实时荧光定量PCR技术，定量测定外周血痕DNA中sjTREC含量，并选择人TATA盒结合蛋白（TATA box binding protein，TBP）序列作为内参基因。采用年龄推断公式Age=-7.181 5 Y-42.458±9.42（Y=dCtTBP-sjTREC）推算血痕的供体年龄，并与供体实际年龄作比较，验证方法准确性。 结果 在收集的30例血痕样品中均能检测到目的基因sjTREC和内参基因TBP。外周血痕sjTREC含量随供体年龄的增长而降低（P＜0.01）。采用本方法推断血痕个体年龄的准确率为76.67%。 结论 利用sjTREC含量推断血痕个体年龄的方法简单、快速、灵敏、种属特异性好，具有重要的应用前景。

关键词: 法医遗传学, 血液, 信号结合T细胞受体删除DNA环, 年龄

Abstract: Objective To determine and verify the correlation formula of age estimation using the content of signal joint T-cell receptor excision DNA circle （sjTREC） in human peripheral blood and to discuss its application value in forensic biological practice. Methods The samples of peripheral blood stains were collected from 30 healthy unrelated individuals whose ages were known. The DNAs were extracted from the samples stored at room temperature after 4 weeks. The content of sjTREC was measured by real-time fluorescent quantitative PCR technique, and the TATA box binding protein （TBP） was selected as reference genes. The age of each sample was predicted with the formula which was Age=-7.181 5 Y-42.458±9.42 （Y=dCtTBP-sjTREC）, and the result was compared with the real age of each individual to determine the accuracy of the formula. Results sjTREC and TBP gene were detectable in all 30 samples of peripheral blood. The contents of sjTREC in human peripheral blood showed a decreasing tendency with aging. The accuracy rate for the age estimation by this method was 76.67%. Conclusion The method for the age estimation with the content of sjTREC was simple, fast, sensitive, and good species specific with important potential application prospect.

Key words: forensic genetics, blood, signal joint T-cell receptor excision DNA circle, age

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