法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (5): 330-332,336.DOI: 10.3969/j.issn.1004-5619.2013.05.003

• 论著 • 上一篇    下一篇

吗啡戒断大鼠中脑腹侧背盖区GAP－43的表达变化

罗良鸣1，2，吴玉锋3，倪维成4，朱  华1   

  1. （1. 温州医学院法医学系，浙江 温州 325035； 2. 温州市公安局刑事科学技术研究所，浙江 温州 325000； 3. 诸暨市公安局刑侦大队，浙江 诸暨 311800； 4. 平阳县公安局刑事技术室，浙江 平阳 325400）
  • 发布日期:2013-10-25 出版日期:2013-10-28
  • 通讯作者: 朱华，男，硕士，副教授，主要从事吸毒的毒理病理和行为学研究；E-mail：wzzhhua@163.com
  • 作者简介:罗良鸣（1978—），男，湖北松滋人，副主任法医师，主要从事法医病理学检验；E-mail：wzrsmart@gmail.com
  • 基金资助:

    浙江省温州市科技局基金资助项目（Y20090237）；浙江省公安厅重大科研资助项目（20110204）

Expression of GAP－43 in Midbrain Ventral Tegmental Area of Morphine Withdrawal Rats

LUO LIANG-MING1,2, WU YU-FENG3, NI WEI-CHENG4, ZHU HUA1   

  1. （1. Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China; 2. Institute of Criminal Science and Technology, Wenzhou Public Security Bureau, Wenzhou 325000, China; 3. Criminal Investigation Team, Zhuji Public Security Bureau, Zhuji 311800, China; 4. Department of Criminal Technology, Pingyang Public Security Bureau, Pingyang 325400, China）
  • Online:2013-10-25 Published:2013-10-28

摘要： 目的 观察吗啡戒断大鼠中脑腹侧背盖区生长相关蛋白-43（growth associated protein-43，GAP-43）在不同时程中的表达，探讨GAP-43在吗啡戒断记忆中的作用。 方法 采用腹腔递增注射盐酸吗啡并自然戒断的方法建立吗啡依赖1周、2周和4周戒断模型，运用免疫组织化学染色观察中脑腹侧背盖区GAP-43的蛋白表达，并用Image-Pro Plus 5.1图像分析软件对结果进行分析。 结果 中脑腹侧背盖区GAP-43的蛋白表达随依赖时间的延长表现为先降低再升高（P<0.01）。 结论 GAP-43可能在中脑腹侧背盖区参与了吗啡戒断记忆。

关键词: 法医病理学, 吗啡依赖, 生长相关蛋白-43, 大鼠

Abstract: Objective To observe the protein expression of growth associated protein-43 （GAP-43） in midbrain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory. Methods Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by immunohistochemical staining and the results were analyzed by Image-Pro Plus 5.1 image analysis system. Results With prolongation of dependent time, the expression of GAP-43 was decreased then increased in midbrain ventral tegmental area. Conclusion GAP-43 could play a role in morphine withdrawal memory in midbrain ventral tegmental area.

Key words: forensic pathology, morphine dependence, growth associated protein-43, rats

中图分类号: