法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (1): 7-12.DOI: 10.3969/j.issn.1004-5619.2014.01.002

• 论著 • 上一篇    下一篇

不同死因大鼠心肌内多种RNA相对表达量变化与PMI的关系

吕叶辉1，张  恒1，潘  晖1，马开军2，李文灿3，陈文峰1，江洁清1，薛爱民1，张  萍4，王慧君4，陈  龙1   

  1. （1. 复旦大学上海医学院法医学系，上海 200032； 2. 上海市公安局刑事科学技术研究所法医室，上海 200043； 3. 上海市公安局浦东分局刑事科学技术研究所，上海 200135； 4. 复旦大学附属儿科医院，上海 201102）
  • 发布日期:2014-02-25 出版日期:2014-02-28
  • 通讯作者: 陈龙，男，博士，主任法医师，副教授，主要从事法医病理学及法医临床学研究；E-mail：chenlong@shmu.edu.cn
  • 作者简介:吕叶辉（1989—），男，山东聊城人，硕士研究生，主要从事法医病理学研究；E-mail：11211010058@fudan.edu.cn
  • 基金资助:

    国家自然科学基金资助项目（81172896）

Relationship between PMI and Relative Expression of Myocardial Various RNAs in Rats Died of Different Causes

LÜ YE-HUI1, ZHANG HENG1, PAN HUI1, MA KAI-JUN2, LI WEN-CAN3, CHEN WEN-FENG1, JIANG JIE-QING1, XUE AI-MIN1, ZHANG PING4, WANG HUI-JUN4, CHEN LONG1   

  1. （1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Department of Forensic Medicine, Institute of Criminal Science and Technology, Shanghai Public Security Bureau, Shanghai 200043, China; 3. Institute of Criminal Science and Technology, Pudong Public Security Subbureau, Shanghai 200135, China; 4. Children’s Hospital of Fudan University, Shanghai 201102, China）
  • Online:2014-02-25 Published:2014-02-28

被引次数

5

摘要： 目的 观察不同死因下大鼠心肌内多种RNA相对表达量随死亡时间（PMI）的变化。 方法 建立断颈死、窒息死和失血性休克死大鼠模型，提取心肌中总RNA，利用实时荧光定量PCR技术检测甘油醛-3-磷酸脱氢酶（glyceraldehyde-3-phosphate dehydrogenase，GAPDH）、β-肌动蛋白（β-actin）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）、缺氧诱导因子-1（hypoxia-inducible factor-1，HIF-1）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6） mRNA以及U6微核RNA（U6 small nuclear RNA，U6 snRNA）的循环阈值，观察各指标相对表达量随PMI的变化情况。 结果 U6 snRNA表达稳定，可作为内对照。在死亡早期，GAPDH、HIF-1、iNOS、TNF-α和IL-6的相对表达量在窒息组和失血性休克组比断颈组呈现不同程度的增加，而β-actin在3个组皆呈现相对表达量下降趋势。在死亡晚期，随着RNA的不断降解，相对表达量持续下降。 结论 死后各RNA相对表达量的特征性变化可为不同死因下的PMI推断提供一定的参考。

关键词: 法医病理学, 心肌, RNA, 死亡时间, 大鼠

Abstract: Objective To observe the changes of relative expression of myocardial various RNAs in rats died of different causes and their relationship with PMI. Methods The rat models were established in which the rats were sacrificed by broken neck, asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium. The quantitative real time PCR was used to calculate threshold cycle values of RNAs including glyceraldehyde-3-phosphate dehydrogenase （GAPDH）, β-actin, inducible nitric oxide synthase （iNOS）, hypoxia-inducible factor-1 （HIF-1）, tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）, and U6 small nuclear RNA （U6 snRNA） and to study the changes of the relative expressions of various indexes with PMI. Results U6 snRNA with stable expression level could be used as appropriate internal control. In the early PMI, the relative expression of GAPDH, HIF-1, iNOS, TNF-α, and IL-6 more characteristically increased in groups of asphyxia and hemorrhagic shock than in group of broken neck, but the quantity of β-actin decreased in all groups. In the late PMI, all the relative expressions significantly declined in correlation with the degradation of RNA. Conclusion The characteristic changes of each RNA expression can be used as references to estimate PMI in deaths by different causes.

Key words: forensic pathology, myocardium, RNA, postmortem interval, rats

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