法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (1): 47-49.DOI: 10.3969/j.issn.1004-5619.2014.01.011

• 技术与应用 • 上一篇    下一篇

16个线粒体DNA SNP复合检测体系的建立

吴  丹1，聂燕钗2，曹  禹1，曹  渊3，周怀谷1   

  1. （1． 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083； 2． 复旦大学上海医学院，上海 200032； 3． 无锡中德美联生物技术有限公司，江苏 无锡 214174）
  • 发布日期:2014-02-25 出版日期:2014-02-28
  • 通讯作者: 周怀谷，男，博士，主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：hgzhou803@hotmail.com
  • 作者简介:吴丹（1972—），女，江苏苏州人，硕士，副主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：wdmd 305@aliyun.com
  • 基金资助:

    公安部科技基础工作专项资助项目（2011GABJC006）

Establishment of the Multiplex Genotyping System for 16 SNP Loci on mtDNA

WU DAN1, NIE YAN-CHAI2, CAO YU1, CAO YUAN3, ZHOU HUAI-GU1   

  1. （1. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. Shanghai Medical College, Fudan University, Shanghai 200032, China; 3. AGCU ScienTech Incorporation, Wuxi 214174, China）
  • Online:2014-02-25 Published:2014-02-28

被引次数

1

摘要：  目的 建立一种复合检测线粒体DNA（mtDNA）单核苷酸多态性（SNP）分型的方法。 方法 通过设计等位基因特异性引物并结合毛细管电泳分型技术平台，建立包含16个mtDNA SNP位点的复合扩增检测体系。对50名汉族无关个体血样进行mtDNA SNP检测，并通过直接测序法对其分型结果进行验证。 结果 50个样本经本检测体系复合扩增后，均得到清晰的SNP分型结果，当模板量在0.5～10 pg时能得到较理想的分型图谱。样本的复合检测结果与直接测序法结果完全一致。 结论 建立的复合检测体系检测mtDNA SNP方法灵敏度高、操作简便、分型准确，为针对mtDNA进行简便、有效的中高通量多态性分型提供了一种新方法。

关键词: 法医遗传学；多态性, 单核苷酸；DNA, 线粒体

Abstract: Objective To establish a multiplex genotyping system of mtDNA SNP. Methods A multiplex analysis system of 16-plex mtDNA SNP loci was established with allele specific PCR and capillary electrophoresis genotyping technology. Fifty samples from unrelated Chinese Han individuals were typed with the multiplex system. The multiplex assay was validated by comparing with the direct sequencing method. Results The genotypes of all 50 samples were correctly determined by the multiplex system. The optimal genotypic graphs were obtained with an input DNA of 0.5-10 pg, and the typing results were completely consistent with those by direct sequencing method. Conclusion The established multiplex system by allele specific PCR has high sensitivity, operational simplicity and high accuracy. It provides an effective and high output method for mtDNA SNP typing.

Key words: forensic genetics, polymorphism, single nucleotide, DNA, mitochondrial

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