法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (1): 44-46.DOI: 10.3969/j.issn.1004-5619.2014.01.010

• 案例分析 • 上一篇    下一篇

1 483例亲子鉴定STR基因座突变的分析

帅  莉1，汪  军2，景  强3，许  彬2，苏  琴2，李芸娟2，李  辉2   

  1. （1. 昆明市延安医院，云南 昆明 650051； 2. 昆明法医院司法鉴定中心，云南 昆明 650031； 3. 昆明医科大学法医学院，云南 昆明 650500）
  • 发布日期:2014-02-25 出版日期:2014-02-28
  • 作者简介:帅莉（1966—），女，四川会东人，副主任技师，主要从事法医物证鉴定和研究；E-mail：1448445238@qq.com

1 483 Cases of Paternity Test with STR Loci Mutation

SHUAI LI1, WANG JUN2, JING QIANG3, XU BIN2, SU QIN2, LI YUN-JUAN2, LI HUI2   

  1. （1. Yan’an Hospital of Kunming, Kunming 650051, China; 2. Center of Forensic Science, Kunming Forensic Hospital, Kunming 650031, China; 3. School of Forensic Medicine, Kunming Medical University, Kunming 650500, China）
  • Online:2014-02-25 Published:2014-02-28

被引次数

16

摘要： 目的 观察和分析PowerPlex?誖18D试剂盒17个STR基因座在云南人群亲子鉴定中的突变现象。 方法 应用Chelex-100法提取DNA，采用PowerPlex?誖18D试剂盒检测1 483例亲子鉴定结论为“肯定”的案例进行基因分型。 结果 1 483例中双亲三联体亲子鉴定1 047例，单亲二联体亲子鉴定436例，总共观察到2 530次减数分裂。1 483例中共观察到24例有1个STR基因座发生突变。在17个STR基因座中观察到11个基因座存在突变现象。 结论 STR基因座突变是较为常见的现象，应不断积累STR基因座突变数据，选择其他多态性好、突变率低的遗传标记，以保证鉴定结果的准确可靠。

关键词: 法医遗传学, 突变, 亲子关系, 云南

Abstract: Objective To observe and analyze the mutation phenomenon of 17 STR loci of PowerPlex?誖18D Kit in paternity test of Yunnan population. Methods The DNA was extracted by Chelex-100 method. The PowerPlex?誖18D Kit was used to test 1 483 cases and their conclusions of paternity tests were verified. Results In the 1 483 cases, 1 047 were parental triplet and 436 were uniparental diad. A total of 2 530 times of meiosis was observed. One STR locus mutation was observed in 24 cases. And 11 mutation loci were found in the 17 STR loci. Conclusion STR loci mutation is a common phenomenon. We should collect the data of STR loci mutation, choose other good polymorphism, low mutation rate of genetic markers, to ensure that the results are accurate and reliable.

Key words: forensic genetics, mutation, parent-child relations, Yunnan

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