法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (1): 50-51,54.DOI: 10.3969/j.issn.1004-5619.2014.01.012

• 技术与应用 • 上一篇    下一篇

改良差异裂解法结合硅珠法提取混合斑精子DNA

韩海军1，2，张玉红2，杨  敏1，2，伊  海1，2，杨耕野2，贾东涛2，卢大儒1   

  1. （1． 复旦大学生命科学学院，上海 200433； 2． 南通市公安局物证鉴定所，江苏 南通 226007）
  • 发布日期:2014-02-25 出版日期:2014-02-28
  • 通讯作者: 卢大儒，男，博士，教授，主要从事人类医学分子遗传学研究；E-mail：drlu@fudan.edu.cn
  • 作者简介:韩海军（1979—），男，江苏如皋人，硕士研究生，主检法医师，主要从事法医物证检验的应用和研究；E-mail：navy_1979@163.com

Extraction of Sperm DNA from Mixed Stain by the Modified Differential Lysis Method Combined with Silicon Bead Method

HAN HAI-JUN1,2, ZHANG YU-HONG2, YANG MIN1,2, YI HAI1,2, YANG GENG-YE2, JIA DONG-TAO2, LU DA-RU1   

  1. （1． School of Life Sciences, Fudan University, Shanghai 200433, China; 2． Institute of Forensic Science, Nantong Public Security Bureau, Nantong 226007, China）
  • Online:2014-02-25 Published:2014-02-28

被引次数

6

摘要： 目的 采用改良差异裂解法结合硅珠法提取混合斑中精子DNA，并评价其应用价值。 方法 收集52例经常规差异裂解法检验含有女性分型的混合斑检材，采用改良差异裂解法结合硅珠法提取精子细胞DNA，IdentifilerTM试剂盒进行PCR扩增检验。并将常规差异裂解法结果作为对照。 结果 52例混合斑检材中，采用改良差异裂解法结合硅珠法检出单一男性精子成分有38例，男性分型检出率达到98.08%。 结论 改良差异裂解法结合硅珠法适合提取混合斑中精子DNA。

关键词: 法医遗传学, 混合斑, 差异裂解法, 硅珠法

Abstract: Objective To extract sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method and to evaluate its application value. Methods Fifty-two mixed stains containing female STR genotypes detected by differential lysis method were collected. The sperm DNA was extracted by the modified method combined with silicon bead method, then genotyped with the IdentifilerTM Kit, and compared with the results of genotyping by the conventional differential lysis method as control. Results Of the 52 samples, 38 samples with sole male STR genotypes in all loci were detected. The detection rate of male STR genotypes was 98.08% through the modified method combined with silicon bead method. Conclusion The modified differential lysis method combined with silicon bead method can be used in extraction of sperm DNA from mixed stain.

Key words: forensic genetics, mixed stain, two-step method, silicon method

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