法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (3): 166-168.DOI: 10.3969/j.issn.1004-5619.2014.03.002

• 论著 • 上一篇    下一篇

大鼠骨骼肌损伤后PMN、MNC及FBC百分率与损伤时间关系

于天水1，2，3，官大威3，赵  锐3，张海东1，2，百茹峰1，2   

  1. （1. 司法文明协同创新中心，北京 100088； 2. 中国政法大学 证据科学教育部重点实验室，北京 100088； 3. 中国医科大学法医学院，辽宁 沈阳 110001）
  • 发布日期:2014-06-25 出版日期:2014-06-28
  • 通讯作者: 官大威，男，教授，博士，博士研究生导师，主要从事法医病理学教学及鉴定工作；E-mail：dwguan@mail.cmu.edu.cn
  • 作者简介:于天水（1982—），男，辽宁大连人，博士，讲师，主要从事法医病理学教学、科研及鉴定工作；E-mail：yutianshui-2000@ qq.com
  • 基金资助:

    国家自然科学基金青年科学基金资助项目（81202383）； 教育部高等学校博士学科点专项科研基金资助项目（2012210 4110025）

Correlation between Percentages of PMN, MNC, FBC and Wound Age after Skeletal Muscle Injury in Rats

YU TIAN-SHUI1,2,3, GUAN DA-WEI3, ZHAO RUI3, ZHANG HAI-DONG1,2, BAI RU-FENG1,2   

  1. （1. Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China; 2. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100088, China; 3. School of Forensic Medicine, China Medical University, Shenyang 110001, China）
  • Online:2014-06-25 Published:2014-06-28

摘要： 目的 研究大鼠骨骼肌机械性损伤后不同时间点多形核白细胞（polymorphonuclear leucocytes，PMN）、单个核细胞（mononuclear cells，MNC）及成纤维样细胞（fibroblastic cells，FBC）百分率的变化。 方法 建立大鼠骨骼肌机械性损伤动物模型，随机分为伤后6 h、12 h、1 d、3 d、7 d、10 d、14 d及正常对照组。应用HE染色法和图像分析检测大鼠骨骼肌损伤后不同时间点PMN、MNC及FBC百分率。 结果 伤后6~12 h，损伤区内可见PMN和MNC浸润，PMN百分率达到峰值；伤后1 d，损伤区内主要以MNC浸润为主，MNC百分率达到峰值，而PMN百分率开始下降；伤后3~7 d，FBC百分率开始逐渐增加，PMN和MNC百分率则逐渐下降；伤后10~14 d，FBC百分率达到峰值。 结论 大鼠骨骼肌损伤区内PMN、MNC及FBC百分率呈时间规律性变化，有望成为骨骼肌损伤时间推断的参考指标。

关键词: 法医病理学, 创伤和损伤, 骨骼肌, 多形核白细胞, 单个核细胞, 成纤维样细胞, 大鼠

Abstract: Objective To study the percentages of polymorphonuclear leukocytes （PMN）, mononuclear cells （MNC） and fibroblastic cells （FBC） in different post-traumatic intervals after skeletal muscle mechanical injury in rats. Methods The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups （6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury） and control group. The percentages of PMN, MNC and FBC in different post-traumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis. Results At post-injury 6-12 h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN decreased. At 3-7 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 10-14 d, the percentage of FBC reached peak. Conclusion The percentages of PMN, MNC and FBC in injured zones showed time-dependent changes, which might be used as reference index for determination of age of skeletal muscle injury.

Key words:  forensic pathology, wounds and injuries, muscle, skeletal, polymorphonuclear leucocytes, mononuclear cells, fibroblastic cells, rats

中图分类号: