法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (4): 241-244.DOI: 10.3969/j.issn.1004-5619.2014.04.001

• 论著 •    下一篇

豚鼠低温死亡血清生化指标的分析

黎世莹1，2，邓恺飞1，邵  煜1，李正东1，秦志强1，陈忆九1，黄  平1   

  1. （1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063； 2. 复旦大学上海医学院法医学系，上海 200032）
  • 发布日期:2014-08-25 出版日期:2014-08-28
  • 通讯作者: 黄平，男，博士，副研究员，副主任法医师，主要从事法医病理学研究；E-mail：huangpingifs@gmail.com 陈忆九，男，研究员，博士研究生导师，主要从事法医病理学研究；E-mail：yijiuchen@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目（81001350）；国家自然科学基金面上基金资助项目（81273339）；上海市自然科学基金资助项目（09ZR1432900，13DZ2271500）

Analysis of Biochemical Markers in Serum of Guinea Pigs after Death Caused by Hypothermia

LI SHI-YING1,2, DENG KAI-FEI1, SHAO YU1, LI ZHENG-DONG1, QIN ZHI-QIANG1, CHEN YI-JIU1, HUANG PING1   

  1. （1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 2. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China）
  • Online:2014-08-25 Published:2014-08-28

被引次数

1

摘要： 目的 检测低温死亡豚鼠的血清生化指标的变化情况，并分析其变化规律，为利用生化指标推断低温死亡提供一定的参考。 方法 20只豚鼠随机分为实验组和对照组，实验组置于-30 ℃左右环境下至死亡，对照组置于25 ℃左右环境下，经相应时间后再断颈处死。分别提取右心血血清，使用生化仪对一系列血清生化指标进行检测。 结果 相较于对照组，低温死亡豚鼠血清中葡萄糖、尿酸、肌酐及尿素氮含量升高（P<0.05），总蛋白和白蛋白含量降低（P<0.05），而其他指标如血清酶和血清离子等则无明显变化。 结论 低温死亡下的血清生化指标的变化存在一定的特征性，有望在低温死亡原因推断中起到辅助鉴定作用。

关键词: 法医病理学, 低温, 血清, 生化指标, 豚鼠

Abstract: Objective To explore the changes and rules of biochemical markers in serum of guinea pigs after death caused by hypothermia and to provide references for fatal hypothermia diagnosis by serum biochemical markers. Methods Twenty guinea pigs were randomly divided into experimental group and control group. The guinea pigs in the experimental group were kept at -30 ℃ until death, while the ones in control group were decapitated after same survival intervals at 25 ℃. The serum was extracted from the whole blood of right ventricular immediately. Subsequently, a series of serum biochemical markers were analyzed by auto bio-chemical analyzer. Results The levels of glucose, uric acid, creatinine and urea nitrogen in the experimental group were significantly higher than those in control group, respectively （P<0.05）. Compared with the control group, the levels of total protein and albumin were significantly lower in the experimental group （P<0.05）. There were no significantly differences of the levels of other markers such as serum enzymes and ions observed between the two groups. Conclusion There are characteristic changes of some specific serum biochemical markers in fatal hypothermia, which may be potentially useful for auxiliary diagnosis of fatal hypothermia.

Key words: forensic pathology, hypothermia, serum, biochemical markers, guinea pigs

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