法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (5): 337-341.DOI: 10.3969/j.issn.1004-5619.2014.05.005

• 论著 • 上一篇    下一篇

30个InDel位点在华东汉族和畲族人群的法医学应用

胡  真1，2，王  正2，张素华2，赵书民2，朱如心2，孙  宽2，李成涛2   

  1. （1． 苏州大学医学部法医学系，江苏 苏州 215123； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2014-10-25 出版日期:2014-10-28
  • 通讯作者: 李成涛，男，博士，研究员，博士研究生导师，主要从事法医遗传学研究；E-mail：lichengtaohla@163.com
  • 作者简介:胡真（1989—），女，浙江金华人，硕士研究生，主要从事法医遗传学研究；E-mail：fly_huzhen@163.com
  • 基金资助:

    国家自然科学基金资助项目（81302026，81222041）； “十二五”国家科技支撑计划项目（2012BAK16B01）

Forensic Application of 30 InDel Loci in Han and She Nationalities of Eastern China

HU ZHEN1,2, WANG ZHENG2, ZHANG SU-HUA2, ZHAO SHU-MIN2, ZHU RU-XIN2, SUN KUAN2, LI CHENG-TAO2   

  1. （1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2014-10-25 Published:2014-10-28

被引次数

5

摘要： 目的 评估Investigator?誖DIPplex试剂盒中30个插入/缺失（insertion/deletion，InDel）多态性位点在华东汉族和畲族人群的法医学应用价值。 方法 采用Investigator?誖DIPplex试剂盒对华东地区565名汉族和119名畲族无关个体进行分型检测，统计分析30个位点的等位基因频率和群体遗传学参数。 结果 在华东汉族人群中平均Ho为0.413 3，平均DP为0.551 1，平均PIC为0.320 0。在畲族人群中平均Ho为0.389 6，平均DP为0.543 3，平均PIC为0.310 0。30个位点在汉族、畲族人群中均符合Hardy-Weinberg平衡（P>0.05）。 结论 Investigator?誖DIPplex试剂盒包含的30个位点在华东汉族和畲族中多态性良好，在特殊亲权鉴定案件中可作为良好的补充体系。

关键词: 法医遗传学, 多态现象, 遗传, 插入/缺失, Investigator?誖DIPplex试剂盒, 汉族, 畲族

Abstract: Objective To evaluate the forensic application value of 30 insertion/deletion （InDel） loci included in Investigator® DIPplex Kit in Han and She nationalities of Eastern China. Methods A total of 565 unrelated individuals in Han nationality and 119 ones in She nationality of Eastern China were investigated using Investigator?誖DIPplex Kit. Allele frequencies, population genetics parameters of the 30 InDel loci were statistically calculated. Results In Han nationality, the mean Ho was 0.413 3, the mean DP was 0.551 1, the mean PIC was 0.320 0. And in She nationality, the mean Ho was 0.389 6, the mean DP was 0.543 3, the mean PIC was 0.310 0. No deviation from Hardy-Weinberg equilibrium was observed in Han and She nationalities （P>0.05）. Conclusion The 30 loci in Investigator?誖DIPplex Kit show good genetic diversity in Han and She nationalities, and could be used as a supplemental tool for some special paternity cases.

Key words: forensic genetics, polymorphism, genetic, InDel, Investigator®DIPplex kit, Han nationality;She nationality

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