法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (6): 463-465.DOI: 10.3969/j.issn.1004-5619.2014.06.015

• 技术与应用 • 上一篇    下一篇

快速溶剂萃取-气相色谱-质谱联用法分析血液中的农药

李文海1，蔺大伟1，孙红雷1，木台力甫·麦麦提吐逊2，王  磊2   

  1. （1. 泰安市公安局刑警支队，山东 泰安 271000； 2. 新疆喀什地区公安处刑侦支队，新疆 喀什 844000）
  • 发布日期:2014-12-25 出版日期:2014-12-28
  • 作者简介:李文海（1974—），男，山东烟台人，高级工程师，主要从事毒物毒品分析研究；E-mail：liwenhai1217@163.com

Analysis of Pesticides in Blood Specimen by GC/MS with Accelerated Solvent Extraction

LI WEN-HAI1, LIN DA-WEI1, SUN HONG-LEI1, MUTAILIFU MAIMAITITUXUN2, WANG LEI2   

  1. （1. Criminal Police Branch, Taian Public Security Bureau, Taian 271000, China; 2. Criminal Investigation Detachment, Kashi Public Security Bureau, Kashi 844000, China）
  • Online:2014-12-25 Published:2014-12-28

摘要： 目的 建立生物检材血液中农药的快速溶剂萃取（accelerated solvent extraction，ASE）法。 方法 通过优化ASE萃取条件，考察萃取温度、时间和萃取剂对回收率的影响，提取血液中的农药进行气相色谱-质谱联用（GC/MS）法定性定量分析。 结果 血液中8种农药的平均回收率在70.6%~92.4%，变异系数小于5.0%，8种农药在0.5~5.0 μg/mL的浓度范围内线性良好。 结论 该方法具有操作简便快捷、回收率高、重现性好等特点，可用于农药的提取检验。

关键词: 法医毒理学, 气相色谱-质谱法, 快速溶剂萃取, 农药, 血液

Abstract: Objective To develop the accelerated solvent extraction （ASE） for determining pesticides present in blood samples. Methods Pesticides were extracted by ASE with optimized parameters to study recovery rate affected by extraction temperature, time and agent. GC/MS was used to perform quantitative analysis．Results The recovery rates of eight pesticides were 70.6%-92.4%. The coefficient of variation was less than 5.0%. A good linear relationship was obtained at the concentration range of 0.5-5.0 μg/mL. Conclusion The method was fast and simple with high recovery rate and good repeatability. It can be applied to analyze pesticides present in the blood specimen.

Key words: forensic toxicology, gas chromatography-mass spectrometry, accelerated solvent extraction, pesticides, blood

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