法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (1): 11-14.DOI: 10.3969/j.issn.1004-5619.2015.01.003

• 论著 • 上一篇    下一篇

大鼠原发性脑干损伤后S100B和GFAP的表达变化

吴雨虹1，王慧君1，王  欣2   

  1. （1. 南方医科大学法医学系，广东 广州 510515； 2. 广州市公安局刑警支队技术所法医检验科，广东 广州 510030）
  • 发布日期:2015-02-25 出版日期:2015-02-28
  • 通讯作者: 王欣，男，硕士，副主任法医师，主要从事法医病理学、损伤检验及现场勘验工作；E-mail：WAXIN@163.com
  • 作者简介:吴雨虹（1983—），女，湖南岳阳人，硕士，主要从事法医病理学研究；E-mail：nyyhwu@163.com

Expression of S100B and GFAP after Primary Brainstem Injury in Rat

WU YU-HONG1, WANG HUI-JUN1, WANG XIN2   

  1. （1. Department of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2. Department of Forensic Medical Examination, Criminal Police Branch of Guangzhou Public Security Bureau, Guangzhou 510030, China）
  • Online:2015-02-25 Published:2015-02-28

被引次数

3

摘要： 目的 观察大鼠原发性脑干损伤后脑干组织中S100B、胶质纤维酸性蛋白（glial fibrillory acidic protein，GFAP）的表达变化，探讨其与脑干损伤时间的变化规律及在损伤中的作用机制。 方法 建立机械性打击脑干损伤动物模型，利用HE染色、Gless嗜银染色和SP免疫组织化学法观察脑干组织中S100B、GFAP在损伤后不同时间的表达，并应用图像分析技术对其进行统计学分析。 结果 S100B阳性表达于伤后30 min开始增多，随时间延长逐渐升高，于24 h达到高峰后开始下降，并于72 h基本降至正常水平；GFAP阳性表达于伤后30 min开始升高，48 h达到高峰后开始下降，但仍高于对照组。 结论 原发性脑干损伤后S100B、GFAP的表达具有时间规律性，在原发性脑干损伤时间的推断及神经修复过程中具有一定的作用。

关键词: 法医病理学, 创伤和损伤, 脑干, S100B, 胶质纤维酸性蛋白, 大鼠

Abstract: Objective To study the expression of S100B and glial fibrillory acidic protein （GFAP） after primary brainstem injury in rat and discuss the changes with brainstern injury time and their mechanism in the injury. Methods The brainstem injury animal model was established using the mechanical impacting method. The HE staining, Gless argentaffin staining and SP immunohistochemical method were applied to observe the changes of S100B and GFAP at different injury time. The immunostaining results were measured statistically with imaging analysis technology. Results A large number of S100B positive cells could be seen in 30 min. Afterward, expression increased gradually with time and peaked up in 24 h, and reversed back the normal in 72 h. The GFAP positive cells showed rise continually in 30 min, and reached the peak in 48 h, then started to decrease, but still higher than that in control. Conclusion The expression of S100B and GFAP is correlated with post traumatic intervals after brainstem injury in rat, and may be useful in estimation post traumatic intervals and nerve regeneration.

Key words: forensic pathology, wounds and injuries, brain stem, S100B, glial fibrillory acidic protein, rats

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