法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (2): 85-87.DOI: 10.3969/j.issn.1004-5619.2015.02.002

• 论著 • 上一篇    下一篇

闭锁小带蛋白-1在脑外伤后皮质中的表达变化

王  涛1，2，孟  颖3，邹冬华1，李正东1，陈忆九1，陶陆阳2   

  1. （1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海200063； 2. 苏州大学医学部法医学系，江苏 苏州 215123； 3. 苏州高新区人民医院ICU，江苏 苏州 215129）
  • 发布日期:2015-04-25 出版日期:2015-04-28
  • 通讯作者: 陈忆九，男，研究员，博士研究生导师，主要从事法医病理学研究：E-mail：chenyj@ssfjd.cn 陶陆阳，男，教授，博士研究生导师，主要从事颅脑损伤及ERP研究；E-mail：luyang.tao@163.com
  • 作者简介:王涛（1984—），男，安徽阜阳人，博士，主要从事颅脑损伤研究；E-mail：0253010217@163.com
  • 基金资助:

    “十二五”国家科技支撑计划项目（2012BAK16B02）；国家自然科学基金资助项目（81273338、81172911）；上海市法医学重点实验室资助项目（14DZ2270800）；上海市博士后科研资助计划项目（14R21423000）；苏州市科技发展计划项目（SZP201304）


Expression of Zonula Occludens-1 in Cerebral Cortex Following Traumatic Brain Injury

（1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, SHANGHAI 200063, CHINA; 2. DEPARTMENT OF FORENSIC MEDICINE, MEDICAL COLLEGE OF SOOCHOW UNIVERSITY, SUZHOU 215123, CHINA; 3. INTENSIVE CARE UNIT, SUZHOU HIGH-TECH ZONE PEOPLE’S HOSPITAL, SUZHOU 215129, CHINA）   

  1. WANG Tao1，2, MENG Ying3, ZOU Dong-hua1, LI Zheng-dong1, CHEN Yi-jiu1, TAO Lu-yang2
  • Online:2015-04-25 Published:2015-04-28

被引次数

3

摘要： 目的 观察闭锁小带蛋白-1（zonula occludens-1，ZO-1）在脑外伤后皮质中不同时段的表达变化。 方法 建立小鼠脑外伤模型，分为脑外伤后1 h、3 h、6 h、12 h、24 h、3 d、7 d组，同时设立假手术组及正常对照组。脑皮质中伊文思蓝（Evans blue，EB）含量检测评估血脑屏障通透性，Western印迹法和免疫组织化学染色法检测损伤皮质区ZO-1的表达。 结果 脑外伤后1 h皮质中EB含量开始增加，伤后1~3 d达高峰，伤后7 d接近正常。Western印迹法显示，ZO-1在损伤1 h后表达下调，损伤1~3 d达到最低值，损伤7 d后明显回升，但仍低于假手术组和正常对照组。免疫组织化学染色显示，正常脑皮质血管中ZO-1呈强阳性表达，损伤后表达逐渐减弱，损伤3 d后阳性表达几乎消失，之后逐渐恢复。 结论 ZO-1在脑外伤后皮质区呈先降低后升高的表达规律，与脑外伤后血脑屏障通透性变化规律呈负相关，为推断脑外伤损伤时间提供了新指标。

关键词: 法医病理学, 脑损伤, 闭锁小带蛋白-1, 小鼠

Abstract: Objective To observe the time-course expression of zonula occludens-1 （ZO-1） in cerebral cortex after traumatic brain injury （TBI）. Methods The TBI model of mouse was established. The mice were divided in 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, 7 d after TBI, sham and control groups. The permeability of the blood brain barrier was evaluated by measuring the extravasation of Evans blue （EB） dye. The expression of ZO-1 in cerebral cortex in the injured area was detected by Western blotting and immunohistochemistry. Results The extravasation of EB dye of injured cortex gradually increased from 1 h, peaked at 1-3 d and approximately decreased to normal at 7 d after TBI. Western blotting revealed that the expression of ZO-1 gradually decreased after 1 h, was at the lowest at 1-3 d, and then significantly increased after 7 d but was still lower than that of normal and sham groups. The result of immunohistochemistry showed that ZO-1 had strong expression in vessel of normal cortex, gradually decreased after TBI, and almost disappeared at 3 d after TBI and gradually recovered to normal level later. Conclusion The expression of ZO-1 in the injured cortex after TBI initially decreases and then increases. The negative correlation between ZO-1 expression and EB extravasation after TBI could be used as a new indicator for wound age estimation.

Key words: forensic pathology, brain injuries, zonula occludens-1, mice

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