法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (4): 284-286.DOI: 10.3969/j.issn.1004-5619.2015.04.008

• 技术与应用 • 上一篇    下一篇

GoldeneyeTM DNA身份鉴定系统25A试剂盒的法医学验证

孙耀东，曹利平   

  1. （盐城市公安局刑事科学技术研究所，江苏 盐城 224002）
  • 发布日期:2015-08-25 出版日期:2015-08-28
  • 作者简介:孙耀东（1975—），男，江苏盐城人，副主任法医师，主要从事法医物证学研究工作；E-mail：syd20163@aliyun.com

Forensic Validation of the GoldeneyeTM DNA ID 25A Kit

SUN YAO-DONG, CAO LI-PING   

  1. （Institute of Criminal Science, Yancheng Public Security Bureau, Yancheng 224002, China）
  • Online:2015-08-25 Published:2015-08-28

摘要： 目的 对GoldeneyeTM DNA身份鉴定系统25A试剂盒的技术性能进行测试及评估。 方法 依据规定的方法对该试剂盒的准确性、灵敏度、一致性、均衡性、稳定性和混合样本等方面进行检验，并应用于部分人员血样和常见案件检材的检验。 结果 各等位基因间均衡性≥42%，阳性DNA样本分型准确，0.125 ng的阳性对照样本可检出完整分型。 结论 GoldeneyeTM DNA身份鉴定系统25A试剂盒可以用于法庭科学的DNA数据库和部分案件的DNA检验。

关键词: 法医遗传学, GoldeneyeTM DNA身份鉴定系统25A试剂盒, 验证

Abstract: Objective To test and estimate the forensic application of GoldeneyeTM DNA ID 25A Kit. Methods The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. Results The peak height balance of the different loci was ≥42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. Conclusion GoldeneyeTM DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.

Key words: forensic genetics, GoldeneyeTM DNA ID 25A Kit, validation test

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