法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (5): 347-351.DOI: 10.3969/j.issn.1004-5619.2015.05.004

• 论著 • 上一篇    下一篇

致死性脑干脑炎婴幼儿炎症细胞分布及PSGL-1的表达

刘  岩1，钟巧娥2，王景舟2，王永在2，顾  捷1，孙文娟1，白慧茹1，陈丽琴1   

  1. （1. 内蒙古医科大学法医学系，内蒙古 呼和浩特 010059； 2. 内蒙古公安厅刑侦总队DNA支队，内蒙古 呼和浩特 010051）
  • 发布日期:2015-10-25 出版日期:2015-10-28
  • 通讯作者: 陈丽琴，女，博士，教授，硕士研究生导师，主要从事法医病理学研究；E-mail：lqchenyj@163.com
  • 作者简介:刘岩（1985—），女，山东济宁人，硕士研究生，主要从事法医病理学研究；E-mail：1615055854@qq.com
  • 基金资助:

    内蒙古自然科学基金资助项目（2013MS1160）；内蒙古高等学校研究项目（NJZY13419）

Distribution of Inflammatory Cells and Expression of PSGL-1 in Infant Brainstem Tissue Related Fatal Brainstem Encephalitis

LIU YAN1, ZHONG QIAO-E2, WANG JING-ZHOU2, WANG YONG-ZAI2, GU JIE1, SUN WEN-JUAN1, BAI HUI-RU1, CHEN LI-QIN1   

  1. （1. Department of Forensic Medicine, Medical University of Inner Mongolia, Hohhot 010059, China; 2. DNA Detachment of Criminal Investigation Team, Public Security Department of Inner Mongolia, Hohhot 010051, China）
  • Online:2015-10-25 Published:2015-10-28

被引次数

1

摘要： 目的 观察手足口病相关脑干脑炎致死的婴幼儿脑干中，炎症细胞分布特征及P选择素糖蛋白配体-1（P-selectin glycoprotein ligand-1，PSGL-1）阳性细胞的分布特点。 方法 收集20例手足口病相关的脑干脑炎死亡婴幼儿脑干组织蜡块作为实验组，10例因非脑部疾病和损伤死亡之婴幼儿脑干组织蜡块为对照组。应用免疫组织化学染色方法，检测多种类型炎症细胞免疫表型标志物和PSGL-1蛋白表达，观察阳性细胞分布特点。 结果 实验组脑干组织炎症细胞“袖套样”浸润的血管周围和胶质小结中，小胶质细胞分布最多，其次是中性粒细胞。小胶质细胞、中性粒细胞和淋巴细胞浸润程度之间差异具有统计学意义（P<0.05）。对照组脑干组织未见炎症细胞“袖套样”浸润的血管和胶质小结。实验组脑干组织PSGL-1广泛表达于炎症细胞，“袖套样”血管周围及胶质小结中阳性细胞数量多并且着色明显。两组的PSGL-1表达量差异具有统计学意义（P<0.05）。 结论 手足口病相关脑干脑炎死亡的婴幼儿脑干中，参与炎性发病过程的主要是小胶质细胞。PSGL-1可能参与手足口病致死性脑干脑炎的疾病过程。

关键词: 法医病理学, 手足口病, 脑干脑炎, 炎症细胞, P选择素糖蛋白配体-1, 婴幼儿

Abstract: Objective To explore the distribution of inflammatory cells and positive expression of P-selectin glycoprotein ligand-1 （PSGL-1） in infant brainstem tissue from hand-foot-mouth disease related fatal brainstem encephalitis. Methods Twenty brainstem samples from infants suffered from brainstem encephalitis were collected as the experimental group. Ten brainstem samples from infants died of non-brain diseases and injuries were collected as the control group. The distribution of inflammatory cells and the expression of PSGL-1 in the two groups were examined by immunohistochemical method. The characteristics of the positive cells were observed. Results In brainstem tissue of the experimental group, there were sleeve infiltrations of inflammatory cells around the vessels and in the glial nodule. Microglia was the most and following was neutrophils around the vessels and in the glial nodule. There was a significant statistical difference among microglias, neutrophils and lymphocytes （P<0.05）. There was no sleeve infiltration in the control group. PSGL-1 protein was expressed widely in inflammatory cells in the experimental group, especially in the inflammatory cells around the vessels and in the glial nodule. But PSGL-1 positive staining could be observed significantly less in the control group comparing with the experimental group （P<0.05）. Conclusion Microglia is the main type of inflammatory cells involved in the progress of the fatal disease. Moreover, PSGL-1 could participate in the pathogenesis of hand-foot-mouth disease related fatal brainstem encephalitis.

Key words: forensic pathology, hand, foot and mouth disease, brain stem encephalitis, inflammatory cell, P-selectin glycoprotein ligand-1, infant

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