法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (1): 45-48.DOI: 10.3969/j.issn.1004-5619.2016.01.010

• 技术与应用 • 上一篇    下一篇

多种遗传标记分析在1例半同胞鉴定中的综合应用

杨  雪1，2，石美森1，2，袁  丽1，2，鲁  涤1，2   

  1. （1. Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China; 2. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100188, China）
  • 发布日期:2016-02-25 出版日期:2016-02-28

Application of Multiple Genetic Markers in a Case of Determination of Half Sibling

YANG XUE1,2, SHI MEI-SEN1,2, YUAN LI1,2, LU DI1,2   

  • Online:2016-02-25 Published:2016-02-28

摘要： 目的 通过对1例半同胞亲缘关系的检验，探讨多种遗传标记在同父异母半同胞关系鉴定中的应用。 方法 分别使用39个常色体STR遗传标记、23个Y染色体STR遗传标记、12个X染色体STR遗传标记对赵3、赵4、赵5与赵1、赵2是否为半同胞亲缘关系进行检验。 结果 依据常染色体、Y染色体及X染色体STR的分型结果，采用家系基因型重建的方法可以推断出赵4（可疑半同胞）与赵1、赵2之间系无关个体，支持赵3（可疑半同胞）、赵5（可疑半同胞）与赵1、赵2为同父异母半同胞关系。 结论 对于同父异母的半同胞鉴定，综合应用多种遗传标记，并采用家系基因型重建的方法进行分析可获得较为可靠的鉴定意见，仅使用ITO法计算半同胞指数或判别函数法分析则难以提供可靠的鉴定意见。

关键词: 法医遗传学, 亲子关系, 同胞关系, 短串联重复序列

Abstract: Objective A case of half sibling was determined with multiple genetic markers, which could be potentially applied for determination of half sibling relationship from same father. Methods Half sibling relationship was detected by 39 autosomal STR genetic markers, 23 Y-chromosomal STR genetic markers and 12 X-chromosomal STR genetic markers among ZHAO-1, ZHAO-2, ZHAO-3, ZHAO-4, and ZHAO-5. Results According to autosomal STR, Y-STR and X-STR genotyping results, it was determined that ZHAO-4 （alleged half sibling） was unrelated with ZHAO-1 and ZHAO-2; however, ZHAO-3 （alleged half sibling）, ZHAO-5 （alleged half sibling） shared same genetic profile with ZHAO-1, and ZHAO-2 from same father. Conclusion It is reliable to use multiple genetic markers and family gene reconstruction to determine half sibling relationship from same father, but it is difficult to determination by calculating half sibling index with ITO and discriminant functions.

Key words: forensic genetics, parent-child relations, sibling relations, short tandem repeat sequences

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