法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (4): 241-244.DOI: 10.3969/j.issn.1004-5619.2016.04.001

• 论著 •    下一篇

MALDI-TOF-IMS分析DAI大鼠脑组织内差异蛋白的分布

任冠恒1，2，翁榕花3，施  妍1，黄  平1，邓恺飞1，刘宁国1，陈忆九1   

  1. （1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台，上海 200063； 2. 南方医科大学基础医学院法医学系，广东 广州 510515； 3. 莆田市公安局城厢分局，福建 莆田 351100）
  • 发布日期:2016-08-25 出版日期:2016-08-28
  • 通讯作者: 刘宁国，男，研究员，主任法医师，主要从事法医病理学研究；E-mail：liuningguo@foxmail.com 陈忆九，男，研究员，博士研究生导师，主要从事法医病理学研究；E-mail：chenyj@ssfid.cn
  • 作者简介:任冠恒（1990—），女，硕士研究生，主要从事法医病理学研究；E-mail：rgh_0101@163.com
  • 基金资助:

    国家自然科学基金资助项目（81273338、81273339、81571851）；中央级科研院所科研专项（GY2015G-2、GY2013Z-3）；上海市科委社会发展专项（14231202500）；上海市司法鉴定专业技术服务平台资助项目（16DZ2290900）

Analysis of Differentially Expressed Proteins Distribution in the Rat Brains with DAI by MALDI-TOF-IMS

REN GUAN-HENG1,2, WENG RONG-HUA3, SHI YAN1, HUANG PING1, DENG KAI-FEI1, LIU NING-GUO1, CHEN YI-JIU1   

  1. （1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 2. Department of Forensic Science, School of Basic Medicine Science, Southern Medical University, Guangzhou 510515, China; 3. Chengxiang Branch of Putian Public Security Bureau, Putian 351100, China）
  • Online:2016-08-25 Published:2016-08-28

摘要： 目的 基于基质辅助激光解吸/电离-飞行时间质谱成像（matrix assisted laser desorption/ionization- time of flight imaging mass spectrometry，MALDI-TOF-IMS）技术建立弥漫性轴索损伤（diffuse axonal injury，DAI）大鼠脑组织内蛋白差异化表达的质谱成像方法。 方法 应用AutoflexⅢ MALDI-TOF质谱仪在质荷比1 000~20 000范围内对DAI组和对照组脑组织进行质谱扫描，利用ClinProTool 2.2软件对两组数据进行统计学分析，挑选出存在表达差异的蛋白质分子进行质谱成像，观察不同质荷比的蛋白质分子在脑组织中的分布情况。 结果 质荷比为4 963、5 634、6 253、6 714及7 532的5个蛋白质分子在DAI大鼠脑组织内表达量存在差异。 结论 MALDI-TOF-IMS可用于大鼠DAI后脑组织内差异蛋白的研究，初步建立了质谱成像技术检测DAI大鼠脑组织中差异蛋白分布的分析方法。

关键词: 法医病理学, 弥漫性轴索损伤, 质谱分析法, 基质辅助激光解吸电离, 脑干, 大鼠

Abstract: Objective To establish the imaging mass spectrometry for analysis of differentially expressed proteins distribution in the rat brains with diffuse axonal injury （DAI） based on matrix assisted laser desorption/ionization-time of flight imaging mass spectrometry （MALDI-TOF-IMS）. Methods MALDI-TOF-IMS scanning were conducted on the brains of DAI group and control group in the m/z range of 1 000 to 20 000 using AutoflexⅢ MALDI-TOF spectrometer. ClinProTool 2.2 software was used for statistical analysis on the data of two groups, and then the differentially expressed proteins were picked out to conduct imaging. The distribution of the proteins with different m/z in the rat brains was observed. Results Five proteins with different m/z, including 4 963, 5 634, 6 253, 6 714 and 7 532, differentially expressed in the rat brains with DAI. Conclusion MALDI-TOF-IMS can be used for studying the differentially expressed proteins in rat brains with DAI and the analysis method is established for exploring the distribution of differentially expressed proteins in the rat brains with DAI using imaging mass spectrometry.

Key words: forensic pathology, diffuse axonal injury, mass spectrometry, matrix-assisted laser desorption- ionization, brain stem, rats

中图分类号: