法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (6): 441-443.DOI: 10.3969/j.issn.1004-5619.2016.06.012

• 技术与应用 • 上一篇    下一篇

COⅠ基因微条形码技术在毛发种属鉴定中的应用

夏  皙，丁  梅   

  1. （中国医科大学法医学院，辽宁 沈阳 110000）
  • 发布日期:2016-12-25 出版日期:2016-12-28
  • 通讯作者: 丁梅，女，教授，主要从事法医遗传学研究；E-mail：dingmei1209@qq.com
  • 作者简介:夏皙（1988—），女，主要从事法医遗传学研究；E-mail：765944359@qq.com

Application of COⅠ Gene Mini-barcoding in Species Identification of Hair

XIA XI, DING MEI   

  1. （School of Forensic Medicine, China Medical University, Shenyang 110000, China）
  • Online:2016-12-25 Published:2016-12-28

被引次数

1

摘要： 目的 利用COⅠ基因微条形码技术对哺乳动物毛发进行种属鉴定。 方法 设计一对哺乳动物COⅠ基因微条形码通用引物，利用共同区PCR技术对来自于哺乳纲5个目11个种属的实验动物毛发DNA进行扩增，并对扩增产物进行双向引物测序，将测序结果进行拼接后所得的基因序列输入BOLD数据库进行同源性比对。 结果 本研究设计的微条形码通用引物能够对所有种属实验动物毛发DNA进行扩增，扩增片段长度147 bp。数据库同源比对结果显示最匹配物种与实验动物种属相符，除狮同源匹配度为98.99%外，其他实验动物同源匹配度均为100%，且狮种内遗传距离1%，种间遗传距离大于种内遗传距离十倍，可以进行种属判定。 结论 建立的COⅠ基因微条形码技术能够快速准确地对哺乳动物毛发检材进行种属鉴定。

关键词: 法医遗传学, 毛发, 种属鉴定, COⅠ基因微条形码

Abstract: Objective To identify the species of mammalian hair using COⅠ gene mini-barcoding technology. Methods A pair of universal primers for mammalian COⅠ gene mini-barcoding were designed. The hair DNA samples of experimental animals from 11 species in 5 orders, mammalia was amplified by PCR technology with universal primers, and the PCR products were sequenced by bi-directional DNA and after the sequences splicing the results were deposited into the BOLD database to perform the homology comparison. Results The DNA of hair from all experiment animal species could be totally amplified by the mini-barcoding universal primers designed in this study. The length of amplified fragment was 147 bp. The result of homology comparison in the database showed that the closest matching species were consistent with the experiment animal species. In addition to the matching degree of Panthera leo （98.99%）, all homology matching degree of the other experiment animals were 100%, and the intraspecific genetic distance of Panthera leo was 1%. The interspecific genetic distance was ten times more than the intraspecific genetic distance which could be used for species identification. Conclusion The COⅠ gene mini-barcode technology is established and can provide fast and accurate species identification for hair of mammals.

Key words: forensic genetics, hair, species identification, COⅠ gene mini-barcoding

中图分类号: