法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (5): 514-515.转521.DOI: 10.3969/j.issn.1004-5619.2017.05.014

• 技术与应用 • 上一篇    下一篇

PrepFiler Express BTATM裂解液联合硅珠法快速提取骨骼DNA

丁少成，张怀才，高林林   

  1. 杭州市公安局刑事科学技术研究所，浙江 杭州 310016
  • 发布日期:2017-10-25 出版日期:2017-10-28
  • 作者简介:丁少成（1981—），男，主检法医师，主要从事法医DNA鉴定及法医病理学检验；E-mail：89781168@qq.com

PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

DING SHAO-CHENG, ZHANG HUAI-CAI, GAO LIN-LIN   

  1. Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310016, China
  • Online:2017-10-25 Published:2017-10-28

摘要： 目的 建立方便、快捷的提取骨骼DNA的方法。 方法 对15份长骨样本清洗消毒后，采用电钻钻取骨屑，使用PrepFiler Express BTATM裂解液进行裂解消化后，应用手工硅珠吸附纯化进行DNA提取检验。 结果 有14份样本获得满意的STR分型，仅1份样本未获得STR分型，检出率为93.33%。 结论 本研究建立的骨骼DNA快速提取方法操作简单、便捷、有效，适应性强，值得推广应用。

关键词: 法医遗传学, 骨骼, DNA, PrepFiler Express BTATM, 硅珠法

Abstract: Objective To establish a convenient and rapid method for extracting DNA from bone. Methods Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTATM lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis. Results STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%. Conclusion The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying.

Key words: forensic genetics, bone, DNA, PrepFiler Express BTATM, silica-extraction method

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