法医学杂志

法医学杂志 ›› 1997, Vol. 0 ›› Issue (2): 68-70+1.

• 论文 • 上一篇    下一篇

扩增X-Y同源Amelogenin基因内含子在性别鉴定中的应用研究

江斌,郭景元,刘超,梁赏猷   

  1. 中山医科大学法医系物证教研室!广东广州,510089,中山医科大学法医系物证教研室!广东广州,510089,广州刑事科学技术研究所!广东,510030,广州刑事科学技术研究所!广东,510030
  • 发布日期:1997-04-25 出版日期:1997-04-28

Determination of sex using PCR of the X-Y homologous gene amelogeuin

JL DU CYJIANG BING,GUO JIN YUAN(DEPARTMENT OF FORENSICMEDICINE.SUN-YAL SEN UNIVERSITY OF MEDICINESCIENCE, 74 ZHONGSHAN 2 ROAD,GUANGZHOU 510089)   

  • Online:1997-04-25 Published:1997-04-28

摘要： 本文用一对针对X－Y同源的Amelogenin基因第一内含子的引物，于同一试管中分别扩增出针对于x和Y染色体的特异性DNA片段：106bp及112bp的PCR产物，经聚丙烯酰胺凝胶电泳后用银染法观察扩增结果，取得了满意的效果．对50pgDNA模板室温放置16年的血痕及单根毛发、烟头等检材均可得到明确的性别鉴定结果．本实验证明该方法简便、灵敏、可靠、特别适用于腐败降解检材的法医学性别鉴定．

关键词: Amelogenin基因, 性别鉴定, 法医学, PCR

Abstract: Sex determination is carried out by simultaneouslyamplifying a X-specific and Y-specific DNA frag-ments which are 106hp and 112hp longrespectively in one PCR reaction using one pair OfPrimers which are SPecific to the first intron Of theX-Y homoIogous gene AmeIogenin.PCR productsare Separated on a PAGE electroPhoresis followedby visulization using silver stain.All the bloodStain.muscle,saliva and hair samples gave correctsex determination.Less than or as little as 50pgtemplate DNA and the 16-year-old blood stain allcan achieve satisfactory amplification.The resultsshowed that the method discribed in this paper is arapid,sensitive,reliable and easily manipulated sextest highly adapted to forensic SPecimen especiallythose petrified and degraded.

Key words: sex determination, amelogenin gene, PCR