法医学杂志 ›› 2002, Vol. 0 ›› Issue (1): 17-18.

• 论文 • 上一篇    下一篇

武汉汉族人群D20S85和D6S477基因座遗传多态性调查

宋海燕,杨荣芝,杨庆恩,余纯应,梅,陈慧   

  1. 华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系 湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,
  • 发布日期:2002-02-25 出版日期:2002-02-28

A study of genetic polymorphism of the STR loci D20S85 and D6S477 in Han population living in Wuhan

SONG HAI-YAN, YANG RONG-ZHI, YANG QING-EN,ET AL. (DEPARTMENT OF FORENSIC BIOLOGY, TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN,HUBEI, 430030 )   

  • Online:2002-02-25 Published:2002-02-28

摘要: 目的对武汉地区280名汉族无关个体的D20S85和D6S477位点遗传多态性进行调查,研究其在法医学检验中的应用价值。方法应用PCR和PAGE技术进行分型检验。结果分别检出10个、9个等位基因,获得各等位基因在该地区汉族人群分布频率,基因型频率分布符合Hardy-Weinberg平衡。两位点的DP值分别为0.9085、0.9127。结论D20S85和D6S477是法医学中重要的遗传标记。

关键词: CR-STR, D20S85, D6S477, 多态性

Abstract: Objective The genetic polymorphism of two STR loci, D20S85 and D6S477, were studied in 280 unrelated Chinese individuals in Wuhan. Methods The PCR amplified products were analyzed by PAGE and silver staining. Results 10 and 9 alleles were observed in these two STR loci, and the discriminating power (DP) were 0.9085 and 0.9127 respectively. No deviations from Hardy-Weinberg equilibrium were found. The two STR loci had been successfully applied to individual identification and paternity testing. Conclusion The results demonstrated that the two loci were useful for forensic identification.

Key words: PCR-STR, D20S85, D6S477, polymorphism