法医学杂志

法医学杂志 ›› 2002, Vol. 0 ›› Issue (3): 150-151+.

• 论文 • 上一篇    下一篇

汉族人群DXS9898基因座的遗传多态性

黄代新,梁伟波,吴梅筠,张林   

  1. 华中科技大学同济医学院法医学系,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院 湖北武汉430030 ,四川成都610041 ,四川成都610041 ,四川成都610041
  • 发布日期:2002-06-25 出版日期:2002-06-28

Genetic polymorphism of the STR DXS9898 locus in Han population

HUANG DAI-XIN 1 ,LIANG WEI-BO 2 ,WU MEI-YUN 2 ,ET AL.(1.FACULTY OF FORENSIC MEDICINE,TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN430030;2.WEST CHINA FORENSIC MEDICINE COLLEGE,SICHUAN UNIVERSITY,CHENGDU610   

  • Online:2002-06-25 Published:2002-06-28

被引次数

5

摘要： 目的研究汉族群体DXS9898基因座的遗传多态性,为法科学应用提供基础数据。方法应用PCR及PAG电泳技术,对成都地区汉族群体199名女性无关个体及97名男性无关个体进行群体遗传学调查。结果共检出6个等位基因,片段大小为189～214bp,其基因型分布符合Hardy-Weinberg平衡。家系调查证实等位基因的传递遵循孟德尔遗传规律。女性样本杂合度为0.5930;男、女性样本个人识别能力(Dp)分别为0.5667、0.9420;父-母-女三联体鉴定的非父排除率(PE)为0.5862。结论DXS9898基因座在法科学个人识别及女性小孩的亲权鉴定中具有较高的实用价值。

关键词: 短串联重复序列, DXS9898, 聚合酶链反应, 多态性

Abstract: Objective Studying the genetic polymorphism of X-STR locus DXS9898in Han population.Methods296unrelated Chinese individuals(199females and97males)living in Chengdu were investigated using PCR and PAG electrophoresis followed by silver staining.Results6alleles were observed and the range of fragment size was189-214bp.The genotype distribution of DXS9898locus was in accordance with Hardy-Weinberg equilibrium.Family survey confirmed Mendelian inheritance of alleles.The observed heterozygosity in females was0.5930,the discriminating power(Dp)were0.5667and0.9420for males and females respectively.The power of exclusion were0.5862and0.4392for trio and duo respectively.Conclusion The results demonstrated that the locus is highly polymorphic and can be used in forensic identification and parentage testing.

Key words: short tandem repeat, DXS9898, polymerase chain reaction, polymorphism