法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (3): 140-142.

• 论文 • 上一篇    下一篇

人脾细胞核几何参数变化与早期死亡时间的相关性研究

舒细记,刘丽江,胡家伟,任亮,庹新兰,刘良   

  1. 江汉大学医学与生命科学学院,江汉大学医学与生命科学学院,武汉市公安局刑侦处,华中科技大学同济医学院法医系,江汉大学医学与生命科学学院,华中科技大学同济医学院法医系 湖北武汉430056华中科技大学同济医学院法医系,湖北武汉430030 ,湖北武汉430056 ,湖北武汉430016 ,湖北武汉430030 ,湖北武汉430056 ,湖北武汉430030
  • 发布日期:2004-06-25 出版日期:2004-06-28

Study on relationship between changes of geometry parameters in human spleen nuclei and the postmortem interval

SHU XI-JI1,3,LIU-LI-JIANG1,HU JIA-WEI2,ET AL.(1.SCHOOL OF MEDICINE﹠LIFE SCIENCES,JIANGHAN UNIVER-SITY,WUHAN430056,CHINA;2.THE DEPARTMENT OF PUBLIC SECURITY,WUHAN430016,CHINA;3.FACULTY OF FOREN-SIC MEDICINE,TONGJI MEDICAL COLLEGE,HUAZHONG UNIVERSITY OF SCI   

  • Online:2004-06-25 Published:2004-06-28

被引次数

13

摘要： 目的运用图像分析技术探讨人脾脏细胞核几何参数变化与死亡时间的关系。方法选31例已知死亡时间的人离体脾组织,在死后36h内每h细胞学涂片、Feulgen-Vans染色,对人脾细胞核的异形指数等5个几何参数进行图像分析。结果脾脏细胞核异形指数在死后5～36h呈上升趋势,r=0.983,线性回归方程y=11.334x-33.339,面积、等效直径、平均直径、周长均与死亡时间无明显的相关性。结论异形指数能较客观地反映人脾细胞核DNA降解后的离散分布状况,与死亡时间有明显相关性,可能是推断早期准确死亡时间的较好形态学指标。

关键词: 法医病理学, 死亡时间, DNA降解, 图像分析

Abstract: Objective Using computer image-analyze technique(CIAT)to study changes of geometry pa-rameters in human spleen nuclei and seek a new experimental method to deduce the estimation the post-mortem interval(PMI).Methods31cadavers that known accurate PMI,sampled and smeared respectively every hour within the first36hours after death,fixed with cold Carony fixation,stained by Feulgen-van's method,and measured5geometry parameters using the image-analyze instrument including Area(A),Mean-Dia(MD),Average Diameter(AD),perimeter(P),Index of density(ID).Results A,MD,AD and P in the human spleen nuclei have no correlation with the PMI.But ID rose regularly with the prolongation of PMI in36hours.There was a definite correlation between ID and the PMI,r=0.983,linear regression equation with PMI(hours)as the dependent variable was calculated for ID.Conclusion Geometry parame-ter ID was proved to be preferable indexes for estimation of PMI in36hours.

Key words: forensic pathology, postmortem interval, DNA degradation, image analysis