法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (3): 160-161.

• 论文 • 上一篇    下一篇

温州汉族人群D7S2846、D19S400和D18S535基因座的遗传多态性研究

张洪勤,吴淑珍,毕云天   

  1. 温州医学院分子生物学研究中心,温州医学院分子生物学研究中心,温州医学院分子生物学研究中心 浙江温州325027 ,浙江温州325027 ,浙江温州325027
  • 发布日期:2004-06-25 出版日期:2004-06-28

A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Han popula-tion in Wenzhou

ZHANG HONG-QIN,WU SHU-ZHEN,BI YUN-TIAN(MOLECULAR BIOLOGY RESEARCH CENTRE OF WENZHOU MEDICAL COL-LEGE,WENZHOU325027,CHINA)   

  • Online:2004-06-25 Published:2004-06-28

被引次数

10

摘要： 目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性。方法EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型。各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938。结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。

关键词: 短串联重复序列, 聚合酶链反应, 遗传多态性

Abstract: Objective To study genetic polymorphism of D7S2846、D19S400and D18S535in Han population in Wenzhou.Methods DNA was extracted with chelex-100method from EDTA-blood samples of194unrelated individuals in Wenzhou and amplified with PCR technique.The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.Results6alleles and15genotypes of D7S2846、10alleles and36genotypes of D19S400、8alleles and26genotypes of D18S535was observed.The heterozygosities of the three loci are0.644,0.724and0.772;The power of discriminating are0.854,0.940and0.938.Conclusion The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium(P>0.05),so the three loci can be used in forensic medicine and in other genetic researches.

Key words: short tandem repeats, polymerase chain reaction, genetic polymorphism