法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (4): 197-199.

• 论文 • 上一篇    下一篇

西安汉族HLA-Cw基因座遗传多态性研究

张洪波,赖江华,赵钧海,李生斌   

  1. 西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室 陕西西安710061 ,陕西西安710061 ,陕西西安710061 ,陕西西安710061
  • 发布日期:2004-08-25 出版日期:2004-08-28

The Study on HLA-Cw Polymorphism from Xi′an Han Population by PCR-sequence Specific Oligonucleotide Probe

ZHANG HONG-BO, LAI JIANG-HUA, ZHAO JUN-HAI, ET AL. (THE NATIONAL LABORATORY OF THE MINISTRY OF HEALTH FOR FORENSIC SCIENCES, XI′AN JIAOTONG UNIVERSITY, XI'AN 710061, CHINA)   

  • Online:2004-08-25 Published:2004-08-28

被引次数

17

摘要： 目的研究西安汉族HLA-Cw基因座遗传多态性,建立西安汉族HLA-Cw基因座基因频率数据库。方法采用序列特异性寡核苷酸探针杂交技术(PCR-SSOP)对130位西安汉族无关个体HLA-Cw基因座进行基因分型。结果共检出16种等位基因,基因频率分布在0.0077～0.1588,其中HLA-Cw*01、03、07基因频率较高,PCR-SSOP分型技术使西安汉族HLA-Cw空白频率降至0.0182。结论西安汉族HLA-Cw基因座基因型分布符合Handy-Weinberg平衡定律,所得到的基因频率数据可为临床器官移植配型、人类学、法医学提供重要的群体遗传学资料。

关键词: HLA-Cw, PCR-SSOP, 多态性, 西安汉族

Abstract: Objective To determine the HLA-Cw allele and genotype frequencies from Xi'an Han population and obtain genetic data. Methods The results of HLA-Cw typing for 130 randomly selected from Xi'an Han population were obtained by using the PCR-Sequence Specific Oligonucleotide Probes (SSOP). Results In this investigation, 16 alleles were detected among 130 unrelated individuals with frequencies from 0.007 7 to 0.158 8 and HLA-Cw*01?03?07 were the most common HLA-Cw alleles. We have made a survey of HLA-Cw alleles frequencies in Xi'an Han Population, with blank frequency being lowered to 0.018 2. Conclusion The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by Hi-square test. The frequency data can be used in forensic and paternity tests, transplant matching and anthropology.

Key words: HLA-Cw, PCR-SSOP, polymorphism, Xi'an Han population