法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (3): 195-196+.

• 论文 • 上一篇    下一篇

扩增TP53内含子8用于生物检材的种属鉴定

王闯,张林,周斌,梁伟波   

  1. 四川大学华西基础医学与法医学院法医物证教研室,四川大学华西基础医学与法医学院法医物证教研室,四川大学华西基础医学与法医学院法医物证教研室,四川大学华西基础医学与法医学院法医物证教研室 四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041
  • 发布日期:2005-06-25 出版日期:2005-06-28

Species Identification of Biological Samples by Amplifying Intron 8 of the TP53 Gene

WANG CHUANG, ZHANG LIN, ZHOU BIN, ET AL. (DEPARTMENT OF FORENSIC BIOLOGY,COLLEGE OF PRECLINICAL ANDFORENSIC MEDICINE,SICHUAN UNIVERSITY,CHENGDU 610041, CHINA)   

  • Online:2005-06-25 Published:2005-06-28

被引次数

11

摘要： 目的扩增常见动物TP53内含子8片段,确定其在法医学生物检材种属鉴定中的应用价值。方法收集标本为包括人在内的15种常见动物的血痕或肌肉组织,提取DNA后定量,应用PCR扩增TP53内含子8,PAGE电泳,银染后观察结果。结果人和猕猴都扩增出一条长度为460bp的片段,鳝鱼、鲢鱼、青蛙、鸭、兔、猫、小白鼠、豚鼠、猪、牛、羊虽有扩增产物,但不在分型区内,鸡、狗未见扩增产物。结论扩增TP53内含子8进行种属鉴定,方法简单,灵敏度较高。

关键词: 肿瘤抑制基因(TP53), 生物检材, 种属鉴定

Abstract: Objective To identify the species of biological samples by amplifying intron 8 of the TP53gene. Methods Collected the bloodstains or muscle tissues of 14 kinds of common animals and human.DNA were quantified after extraction.The PCR amplification products were analyzed by PAGE and silverstaining. Results The human and monkey have one amplification band of 460bp while the eel,fish,frog,duck,rabbit,cat,mouse,cavy,pig,ox and sheep could amplify different unspecial bands.No amplificationproducts were found in chicken and dog. Conclusion The method of species identification by amplifyingintron 8 of the TP53 gene is simple and sensitive.

Key words: TP53 tumor suppressor gene, biological samples, species identification