法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 93-94.

• 论文 • 上一篇    下一篇

潍坊地区六种常见嗜尸性蝇类mtDNA COI区序列研究

王新杰;王学海;刁立江;吕桂平;   

  1. 潍坊市公安局,潍坊市公安局,潍坊市公安局,山东省公安厅 山东潍坊261061,山东潍坊261061,山东潍坊261061,山东济南250001
  • 发布日期:2006-04-25 出版日期:2006-04-28

Identification of Six Species Of Sarcosaphagous Flies(Diptera) by Sequence Analysis of Cytochrome Oxidase Subunit I Gene (COI ) in Weifang

WANG XIN-JIE1, WANG XUE-HAI1, DIAO LI-JIANG1, LU GUI-PING2 (1.PUBLIC SECURITY BUREAU OF WEIFANG MUNICIPALITY, WEIFANG 261061, CHINA ; 2.DEPARTMENT OF PUBLIC SECURITY OF SHANDONG PROVINCE, JINAN 250001, CHINA)   

  • Online:2006-04-25 Published:2006-04-28

被引次数

42

摘要： 目的解决蝇类成虫、蛹和蛆快速、准确鉴定的难题。方法随机采集潍坊地区人尸周围环境中的蝇类成虫、蛹和蛆,提取其mtDNA,经PCR扩增、产物检测与纯化、测序等,比较其序列差异。结果六种常见嗜尸性蝇类相互之间序列差异明显,同一种蝇的成虫、幼虫、蛹之间未见差异。结论mtDNACOI区序列分析可作为法医学嗜尸性蝇类种属鉴别的可靠方法。

关键词: 嗜尸性蝇类, 种属鉴别, 线粒体DNA细胞色素氧化酶辅酶I, 法医学

Abstract: Objective To identify sarcosaphagous flies and their larvae, pupa. Methods sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing. Results The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples. Conclusion It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304 bp region of the COI in mtDNA.

Key words: Sarcosaphagous flies, species identification, mtDNA, COI, forensic