法医学杂志

法医学杂志 ›› 2006, Vol. 22 ›› Issue (5): 353-354.

• 论文 • 上一篇    下一篇

固相萃取毛细管区带电泳法测定全血中甲基苯丙胺

龚飞君;张润生;   

  1. 上海市刑事科学技术研究所,上海市刑事科学技术研究所 上海200083,上海200083
  • 发布日期:2006-10-25 出版日期:2006-10-28

Determination of Methamphetamine in Whole Blood by Capillary Zone Electrophoresis after Solid Phase Extraction

GONG FEI-JUN, ZHANG RUN-SHENG (SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY, SHANGHAI 200083, CHINA)   

  • Online:2006-10-25 Published:2006-10-28

被引次数

24

摘要： 目的建立全血中甲基苯丙胺的固相萃取毛细管区带电泳法检验方法。方法血液采用OasisHLB固相萃取小柱直接萃取,BeckmanP/ACEMDQ毛细管电泳仪区带法分析。结果回归方程y=0.0083x-0.0164,线性范围5～75μg/mL(r=0.994),日内精密度RSD=2.22%,日间精密度RSD=5.31%,盐酸甲基苯丙胺质量浓度为25.0μg/mL的全血相对回收率(91.63±2.5)%。电泳分离良好,空白无干扰。结论本方法操作简便,适用于全血中甲基苯丙胺的检验。

关键词: 甲基苯丙胺, 全血, 固相萃取, 毛细管区带电泳法

Abstract: Objective To develop a specific CZE method for the determination of methamphetamine in whole blood after solid phase extraction. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood and the sample was analysized by CZE. Results The method showed excellent linearity and the linear correlation coefficient was 0.994. The relative standard deviation for between-day and within-day were 5.31% and 2.22%, respectively. Conclusion The method is effective, simple, reliable and has been used in the determination of methamphetamine in whole blood.

Key words: methamphetamine, whole blood, SPE, CZE