法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (5): 342-344.

• 论文 • 上一篇    下一篇

X染色体STR单倍型DXS7424-DXS101遗传多态性

余兵;托娅;桂宏胜;李生斌;   

  1. 西安交通大学卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室,西安交通大学卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室,西安交通大学卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室,西安交通大学卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室 陕西西安710061,陕西西安710061,陕西西安710061,陕西西安710061
  • 发布日期:2007-10-25 出版日期:2007-10-28

Validation the Haplotype Polymorphisms of the DXS7424-DXS101 on X-chromosome

YU BING,TUO YA,GUI HONG-SHENG,LI SHENG-BIN (KEY LABORATORY OF FORENSIC SCIENCES,NATIONAL MINISTRY OF HEALTH; KEY LABORATORY OF MINISTRY OF EDUCATION FOR ENVIRONMENT AND GENES RELATED TO DISEASES,XI’AN JIAOTONG UNIVERSITY,XI’AN,710061 CHINA)   

  • Online:2007-10-25 Published:2007-10-28

被引次数

6

摘要： 目的研究X染色体STR单倍型DXS7424-DXS101在汉族群体中的法医学特征及其分布特点。方法本实验应用PCR和聚丙烯酰胺凝胶电泳及银染技术对DXS7424和DXS101位点进行基因分型,利用相关统计方法进行数据分析。结果汉族151个男性样本中共检出37种单倍型,频率分布在0.0066～0.1391,GD值为0.9453,DP值达到0.9389,最常见单倍型为16-23。结论DXS7424-DXS101作为单倍型分析在汉族群体中具有较高的个体识别率和非父排除率,在法医学特殊亲权鉴定中具有良好的应用前景。

关键词: X染色体, 短串联重复序列(STR), 单倍型, 多态性

Abstract: Objective To validate the genetic characteristics and distribution of DXS7424-DXS101 on X chromosome in Han population. Methods DXS7424 and DXS101 loci were genotyped by PCR,PAGE and silvers stain methods. Their genetic parameters were analyzed by Arlequin software. Results There were 37 haplotypes detected in 151 Han unrelated males. The frequencies ranged from 0.006 6 to 0.139 1,with a GD value of 0.945 3 and a DP value of 0.938 9. Haplotypes 16-23 were the most common haplotypes in Han population. Conclusion Analysis of combined DXS7424 and DXS101 haplotypes appears to be a powerful means in population genetics and forensic practice for determination of identity and paternity.

Key words: X chromosome, short tandem repeats (STR), haplotype, polymorphism