法医学杂志

法医学杂志 ›› 2007, Vol. 23 ›› Issue (4): 292-294.

• 论文 • 上一篇    下一篇

尿液中丁丙诺啡的提取方法

刘冬娴;贺江南;   

  1. 湖南公安高等专科学校刑事技术系,湖南公安高等专科学校刑事技术系 湖南长沙410138,湖南长沙410138
  • 发布日期:2007-08-25 出版日期:2007-08-28

A Study in Extraction Method of Buprenorphine in Urine

LIU DONG-XIAN,HE JIANG-NAN(DEPARTMENT OF CRIMINAL TECHNOLOGY,HUNAN PUBLIC SECURITY COLLEGE,CHANGSHA 410138,CHINA)   

  • Online:2007-08-25 Published:2007-08-28

被引次数

7

摘要： 目的建立尿液中丁丙诺啡提取方法。方法尿液加pH 7.0缓冲液后,用三氯甲烷进行液相萃取或加pH 10.8缓冲液后,用401有机担体作吸附剂、三氯甲烷作洗脱剂进行固相萃取,GC/NPD法检测。结果尿液中丁丙诺啡液相萃取萃取率可达86.6%以上,固相萃取可达83.0%以上。结论两种提取方法萃取率高,可用于尿液中丁丙诺啡的提取。

关键词: 丁丙诺啡, 液相萃取, 固相萃取, 尿液

Abstract: Objective The extraction method for separating buprenorphine from urine was described.Methods Buprenorphine was extracted with chloroform at pH7 or with SPE(extracted by organic support 401 at pH10.8,then eluted with chloroform),finally determined by GC-NPD.Results The extracted yields of the analyte in specimens were 86.6% by solution-phase extraction and 83.0% by solid-phase extraction(SPE) respectively.Conclusion These two methods are simple and accurate for separating buprenorphine from urine.

Key words: buprenorphine, solid-phase extraction, solution-phase extraction, urine