法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (1): 34-37.

• 论文 • 上一篇    下一篇

签字笔上附着的脱落上皮细胞STR分型

杨帆;梅善宗;李永宏;冯燕;俞卫东;张越;   

  1. 皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系,南京市公安局法医中心,南京市公安局法医中心,皖南医学院法医学系 安徽芜湖241001,安徽芜湖241001,安徽芜湖241001,江苏南京210000,江苏南京210000,安徽芜湖241001
  • 发布日期:2008-02-25 出版日期:2008-02-28

STR Genotyping from Trace Epithelial Cells on Fountain Pen

YANG FAN1, MEI SHAN-ZONG1, LI YONG-HONG1, FENG YAN2, YU WEI-DONG2, ZHANG YUE1 (1.DEPARTMENT OF FORENSIC MEDICINE, WANNAN MEDICAL UNIVERSITY, WUHU 241001, CHINA;2.CENTER OF FORENSIC IDENTIFICATION, NANJING PUBLIC SECURITY BUREAU, NANJING 210000, CHINA)   

  • Online:2008-02-25 Published:2008-02-28

被引次数

10

摘要： 目的探讨遗留在签字笔上微量脱落细胞DNA分型的可行性以及保存时间对分型的影响。方法17名志愿者每人使用7支签字笔,每支笔每天使用20min,为期1个月,分别保存1、3、5、7、14、21和28d,运用硅珠法提取签字笔上微量脱落细胞中的DNA,应用荧光标记PCR-STR技术进行DNA分型,同时采集上述17名志愿者口腔拭子作为对照,分析签字笔作为检材进行DNA分型的可行性以及保存时间对DNA分型的影响。结果以基因座检出个数为指标,签字笔脱落细胞和口腔拭子的DNA分型结果随保存时间变化而产生的差异具有统计学意义(P<0.01)。签字笔保存1、3、5、7、14、21和28d后进行DNA分型检出的基因座个数与对应的口腔拭子DNA分型检出的基因座个数相比差异均有统计学意义(P<0.01)。签字笔使用后保存1d进行DNA分型,可明确判读12个以上基因座的占41.2%。结论签字笔上附着的微量手指脱落细胞可作为一种法庭生物检材进行DNA分型,但其保存时间会影响DNA分型。

关键词: 法医物证学, 上皮细胞, DNA, STR复合扩增

Abstract: Objective To evaluate the feasibility of STR genotyping from trace epithelial cells on fountain pen and to discuss the impact of conservation time on DNA typing. Methods Seven fountain pens were separately used by each of the 17 volunteers 20 minutes per day for a month and then were preserved on day 1, 3, 5, 7, 14, 21, and 28. DNA was extracted from the epithelial cells on fountain pen by silicon bead and was genotyped by Identifier kit. The corresponding control samples were buccal swabs of the above volunteers. The detectable numbers of loci were counted for assessment. Results There were statistically significant differences in the DNA genotyping by detectable numbers of gene loci between buccal swabs and epithelial cells on fountain pen of different conservation times (P<0.01). The differences of detectable numbers of loci between the epithelial cells on fountain pen preserved on day 1, 3, 5, 7, 14, 21, 28 and the corresponding oral swabs were also statistically significant (P<0.01). More than 12 loci could be successfully genotyped in 41.2% samples from the epithelial cells on fountain pen if the tests were performed within 24 hours. Conclusion The trace epithelial cells on fountain pen can be used as biological samples for personal identification, but the conservation time would have influence on the results of DNA genotyping.

Key words: Forensic biology, epithelial cells, DNA, STR multiplex amplification