法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (3): 161-164.

• 论文 • 上一篇    下一篇

大鼠死后脑组织的傅里叶变换红外光谱变化

柯咏;张建钢;黄平;鲁庆阳;樊栓良;徐永城;王振原;   

  1. 西安交通大学医学院法医学系,西安市公安局刑事侦察局技术处,西安交通大学医学院法医学系,西安交通大学医学院法医学系,西安交通大学医学院法医学系,西安交通大学医学院法医学系,西安交通大学医学院法医学系 陕西西安710061,陕西西安710016,陕西西安710061,陕西西安710061,陕西西安710061,陕西西安710061,宁波市公安局鄞州分局,浙江宁波315100,陕西西安710061
  • 发布日期:2008-06-25 出版日期:2008-06-28

The Changes of Fourier Transform Infrared Spectroscopy in Rat's Brain

KE YONG1, ZHANG JIAN-GANG2, HUANG PING1, LU QING-YANG1, FAN SHUAN-LIANG1, XU YONG-CHENG1,3, WANG ZHEN-YUAN1 (1. DEPARTMENT OF FORENSIC MEDICINE, MEDICAL COLLEGE, XI′AN JIAOTONG UNIVERSITY, XI′AN 710061, CHINA; 2. TECHNICAL SURVEILLANCE DIVISION, XI′AN PUB   

  • Online:2008-06-25 Published:2008-06-28

被引次数

29

摘要： 目的应用傅里叶变换红外(Fourier transform infrared,FTIR)光谱技术分析大鼠死后脑组织随死亡时间推移的化学变化过程,为死亡时间推断的研究提供新的途径与数据。方法大鼠断颈处死后置于(30±2)℃环境下,在不同时间点提取大鼠脑皮质,运用FTIR光谱仪检测不同化学基团的变化。结果随着死亡时间的推移,大鼠脑组织FTIR光谱的主要吸收峰峰位没有明显变化,而其峰强有明显差异:(1)与核酸有关的谱带的相对峰强呈明显下降趋势;(2)酰胺Ⅰ、Ⅱ的峰强比(I_(1647)/I_(1541))呈下降趋势;(3)1456和1398cm~(-1)谱带的峰强各自呈下降和上升趋势;(4)2852、2871、2923和2958cm~(-1)谱带的峰强相对于1647cm~(-1)而言,呈<正>上升趋势。结论脑组织可以作为FTIR光谱技术分析死亡时间的适用检材。

关键词: 法医病理学, 傅里叶变换红外, 死亡时间, 脑组织, 化学基团

Abstract: Objective To apply Fourier transform infrared (FTIR) spectroscopy to study the process of postmortem degradation of the rat brain and to provide a new way for the estimation of postmortem interval (PMI). Methods The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at (30±2)℃. To measure the content of the chemical groups in postmortem rat brains at the different time points from 0 to 36 h using the FTIR spectrograph. Results With prolongation of PMI, the peak position of main absorbance bands in the FTIR spectra showed no significant changes, while the peak levels showed dramatic changes: (1) The relative peak intensity of 1080 cm-1, 1238 cm-1 (I1080/I1398,I1238/I1398) associated with nucleic acid decreased obviously; (2) The peak intensity ratio at Amide I, II (I1647/I1541) decreased; (3) The peak intensities at 1456 cm-1 and 1398 cm-1 showed a decreased and an increased trend, respectively; (4) Compared to the peak intensity of 1647 cm-1, the peak intensities at 2852 cm-1, 2871 cm-1, 2923 cm-1, and 2958 cm-1 tended to increase, with only a slightly increased tendency in peak intensity of 2871 cm-1. Conclusion FTIR spectroscopy may be potentially used as an effective method for estimating the PMI in medicolegal practice using brain tissue sample.

Key words: fourier transform infrared, postmortem interval, brain, chemical group