法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (3): 172-174.

• 论文 • 上一篇    下一篇

大鼠弥漫性轴索损伤后NGF表达

陈宗云;汪静宇;李永宏;   

  1. 皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系 安徽芜湖241001,安徽芜湖241001,安徽芜湖241001
  • 发布日期:2008-06-25 出版日期:2008-06-28

The Expression of NGF after Rat Diffuse Axonal Injury

CHEN ZONG-YUN, WANG JING-YU, LI YONG-HONG (DEPARTMENT OF FORENSIC MEDICINE, WANNAN MEDICAL COLLEGE, WUHU 241000, CHINA)   

  • Online:2008-06-25 Published:2008-06-28

被引次数

10

摘要： 目的研究大鼠弥漫性轴索损伤后神经生长因子(NGF)表达的时间变化规律及意义。方法采用SD雄性大鼠复制弥漫性轴索损伤(DAI)模型,分别于伤后l、3、6、12、24、48h和3、7d取脑组织,应用免疫组织化学法对DAI后不同时间段大脑皮层、丘脑、小脑和海马部位NGF的表达变化进行观察并与正常组、假手术组对照。结果正常及假手术组大鼠大脑皮层、丘脑、小脑、海马均有少量NGF的表达。打击后1hNGF表达增强,12h达高峰,3d开始下降,7d降至正常。结论NGF参与弥漫性轴索损伤后的修复;NGF的时序性变化有望成为法医学脑损伤时间推断的客观指标。

关键词: 法医病理学, 免疫组织化学, 神经生长因子, 弥漫性轴索损伤, 损伤时间

Abstract: Objective To study the expression of nerve growth factor (NGF) in diffuse axonal injury (DAI) in rat. Methods Eighty SD rats were used and samples were taken at l h, 3 h, 6 h, 12 h, 24 h, 48 h, 3 d, and 7 d after brain injury. The expressions of NGF in cerebral cortex, thalamus, cerebellum, and hippocampus were studied with immunohistochemistry and compared with normal group and sham operation group. Results Low expression of NGF was observed in normal group and sham operation group. The expression of NGF increased 1h after injury, peaked at 12 h, and returned to basal level at day 7. Conclusion NGF is involved in repair of DAI. The changes of NGF expression following DAI may be applied to estimate the post-injury time interval of the brain in forensic medicine.

Key words: forensic pathology, immunohistochemistry, NGF, diffuse axonal injury, injury time