法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (6): 407-410.

• 论文 • 上一篇    下一篇

大鼠皮肤和肌肉挫伤后细胞间黏附分子-1 mRNA表达变化

孙俊红;杜秋香;王小伟;王英元;   

  1. 山西医科大学法医学院;
  • 发布日期:2008-12-25 出版日期:2008-12-28

Changes of ICAM-1 mRNA Expression in Rats′ Skin and Muscle after Contusion

SUN JUN-HONG, DU QIU-XIANG, WANG XIAO-WEI, WANG YING-YUAN (SCHOOL OF FORENSIC MEDICINE, SHANXI MEDICAL UNIVERSITY, TAIYUAN 030001, CHINA)   

  • Online:2008-12-25 Published:2008-12-28

被引次数

5

摘要： 目的应用实时荧光定量PCR技术检测大鼠皮肤和肌肉挫伤后组织中细胞间黏附分子-1(intercel-lular adhesion molecular-1,ICAM-1)mRNA的表达,分析其与损伤时间的关系。方法制备大鼠挫伤模型,于伤后0.5、1、6、12、18、24和30h取材,一步法提取总RNA,检测其完整性和质量浓度,逆转录合成第一链cDNA,以rpl32为内参基因进行荧光定量检测,采用△△Ct法比较其与正常皮肤、肌肉组织中ICAM-1mRNA表达的倍数关系。结果皮肤挫伤后ICAM-1mRNA的表达0.5h达到峰值,24h下降至正常对照组的7倍,随后再次升高;在肌肉组织中于损伤后0.5h表达显著增强,6h达到峰值,18h降至最低后再次升高。结论大鼠皮肤、肌肉挫伤后ICAM-1mRNA表达随损伤时间呈规律性变化,可望用于早期损伤时间推断。

关键词: 法医病理学, 挫伤, 细胞间黏附分子, 实时荧光定量聚合酶链式反应, 损伤时间推断

Abstract: Objective To investigate the expression of intercellular adhesion molecule-1(ICAM-1) mRNA in contused skin and muscle of rats and the relationship between the ICAM-1 expression and the wound age. Methods The samples were taken at 0.5, 1, 6, 12, 18, 24 and 30 h after contusion of rats, respectively. Total RNA was extracted both from the skin and muscle samples of each group and reverse transcription polymerase chain reaction (RT-PCR) was conducted to synthesize the 1st strand cDNA. The amount of ICAM -1 mRNA in each sample was quantified using rp132 intrinsic fluorescent assay and compared by the 2(-Delta Delta Ct) method with that from control samples. Results After contusion the expression of ICAM-1 mRNA in skin increased rapidly and peaked at 0.5 h, at 24 h degraded to the amount that was seven times as much as the control group, then rised again. The expression of ICAM-1 mRNA in muscle increased significantly within 0.5 h and peaked at 6 h, reached the minimum at 18 h, then increased again. Conclusion It is suggested that ICAM-1 mRNA analysis may be useful for estimation of early wound age because of its time-related expression after contusion in skin and muscle.

Key words: forensic pathology, contusion, intercellular adhesion molecular, real-time fluorescence quantitative PCR, wound age estimation