法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (2): 115-117+.

• 论文 • 上一篇    下一篇

测序方法验证12个STR基因座的基因型

李成涛;赵珍敏;柳燕;李莉;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学生命科学学院;
  • 发布日期:2009-04-25 出版日期:2009-04-28

Genotype Validation of 12 STR Loci by Sequencing Method

LI CHENG-TAO1,2, ZHAO ZHEN-MIN1, LIU YAN1, LI LI1 (1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R. CHINA, SHANGHAI 200063, CHINA; 2. SCHOOL OF LIFE SCIENCES, FUDAN UNIVERSITY, SHANGHAI 200433, CHIN   

  • Online:2009-04-25 Published:2009-04-28

摘要： 目的用测序方法验证12个STR基因座的基因型。方法根据各STR基因座的特异性序列,对CSF1PO、FGA、TH01、TPOX、VWA、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51和D21S1112个STR基因座设计了PCR引物并对标准品9947A和突变样本进行PCR产物测序。结果标准品9947A和突变样本的测序结果均与其基因分型结果一致。结论建立的测序方法准确、灵敏,可以用于STR基因型的确认。

关键词: 法医遗传学, 短串联重复序列, 突变

Abstract: Objective To validate the genotype of 12 STR loci by sequencing method. Methods According to the special sequence of 12 STR loci(CSF1PO, FGA, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the relative PCR primers were designed. PCR products of control DNA(9947A) and STR mutation samples were sequenced. Results The sequencing results of both the control DNA(9947A) and STR mutation samples were consistent with their genotyping results. Conclusion The sequencing method developed are accurate and sensitive, can be used for the validation of STR genotyping results.

Key words: forensic genetics, short tandem repeat, mutation