法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (3): 161-163.

• 论文 • 上一篇    下一篇

大鼠骨骼肌挫伤后细胞间黏附分子-1 mRNA表达变化

裴明;孙俊红;高彩荣;   

  1. 山西医科大学法医学院;徐州市公安局泉山分局;
  • 发布日期:2009-06-25 出版日期:2009-06-28

Changes of ICAM-1 mRNA Expression in Rats' Skeletal Muscle after Contusion

PEI MING1,2,SUN JUN-HONG1,GAO CAI-RONG1(1.SCHOOL OF FORENSIC MEDICINE,SHANXI MEDICAL UNIVERSITY,TAIYUAN 030001,CHINA;2.QUANSHAN BRANCH OF XUZHOU PUBLIC SECURITY BUREAU,XUZHOU 221008,CHINA)   

  • Online:2009-06-25 Published:2009-06-28

被引次数

11

摘要： 目的应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术检测大鼠骨骼肌挫伤后细胞间黏附分子-1(intercellular adhesion molecular-1,ICAM-1)mRNA表达,分析其与挫伤时间的关系。方法建立大鼠骨骼肌挫伤模型,分别取伤后0.5、1、6、12、18、24、30h及对照组检材。检材冰冻切片后进行FISH,激光共聚焦显微镜观察。结果大鼠骨骼肌挫伤后ICAM-1 mRNA的表达6h达到峰值,18h下降至对照组的3.46倍,随后继续升高。结论大鼠骨骼肌挫伤后30h内ICAM-1 mRNA的表达有一定的时间规律性,可望作为推断骨骼肌早期损伤时间的指标之一。

关键词: 法医病理学, 挫伤, 细胞间黏附分子, 原位杂交, 荧光, 损伤时间的推断, 大鼠

Abstract: Objective To investigate the expression of intercellular adhesion molecular-1(ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization(FISH) and its relationship with the contusion interval.Methods To make the contusion models with rats skeletal muscle.The samples were taken to extract mRNA at 0.5 h,1 h,6 h,12 h,18 h,24 h,30 h and from the control group after contusion respectively.FISH was performed on frozen section samples and the sections were observed using LSCM.Results The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion.Its level fell to 3.46 times the level of control group at 18 h and then increased again.Conclusion The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.

Key words: forensic pathology, contusion, intercellular adhesion molecules, in situ hybridization, fluorescence, wound age estimation, rats