法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (3): 164-167.

• 论文 • 上一篇    下一篇

过敏性休克死亡者肺组织类胰蛋白酶及类糜蛋白酶的表达

卫志燕;高彩荣;裴明;   

  1. 山西医科大学法医学院法医病理学教研室;
  • 发布日期:2009-06-25 出版日期:2009-06-28

Expression of Tryptase and Chymase in Human Lung Tissue of Anaphylactic Shock

WEI ZHI-YAN,GAO CAI-RONG,PEI MING(DEPARTMENT OF FORENSIC PATHOLOGY,SCHOOL OF FORENSIC MEDICINE,SHANXI MEDICAL UNIVERSITY,TAIYUAN 030001,CHINA)   

  • Online:2009-06-25 Published:2009-06-28

被引次数

23

摘要： 目的观察类胰蛋白酶与类糜蛋白酶在过敏性休克死亡人体肺组织中的表达,并探讨其在过敏性休克死亡法医学鉴定中的意义。方法应用荧光免疫组化法对类胰蛋白酶与类糜蛋白酶进行检测,同时以10例CO中毒死者为对照;采用图像分析仪及Image-pro plus 5.0.2软件进行图像分析,计算阳性肥大细胞数及类胰蛋白酶、类糜蛋白酶荧光表达强度。结果与对照组相比,实验组肺组织中类胰蛋白酶和类糜蛋白酶表达差异有统计学意义(P<0.05)。结论过敏性休克死亡人体肺组织中类胰蛋白酶与类糜蛋白酶的表达增强,可作为过敏性休克死亡的形态学诊断依据。

关键词: 法医病理学, 过敏反应, 肺, 类胰蛋白酶类, 糜蛋白酶类

Abstract: Objective To explore the expression of tryptase and chymase in human lung tissue of anaphylactic shock and its value for forensic medicine.Methods With ten carbon monoxide poisoning cases as control group,the levels of tryptase and chymase were observed by immunofluorescence and analyzed using the Image Analyze and the Image-pro plus 5.0.2.The positive mast cells were counted and the levels of the tryptase and chymase were calculated respectively.Results There was a statistically significant difference(P<0.05) for the tryptase and chymase concentrations in the lung tissue between the anaphylactic shock group and the control group.Conclusion The levels of the tryptase and the chymase expression are greatly increased in human lung tissue of anaphylactic shock,which may provide the morphological evidence and reference for the diagnosis of anaphylactic shock in forensic practice.

Key words: forensic pathology, anaphylaxis, lung, tryptases, chymases