法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (1): 22-25.

• 论文 • 上一篇    下一篇

TaqMan探针技术用于X-SNP位点的分型

张素华;李莉;李成涛;赵书民;   

  1. 苏州大学医学部法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室;
  • 发布日期:2010-02-25 出版日期:2010-02-28

X-SNP Genotyping Using the TaqMan Probe Technology

ZHANG SU-HUA1,2,LI LI2,LI CHENG-TAO2,ZHAO SHU-MIN2(1.DEPARTMENT OF FORENSIC MEDICINE,MEDICAL COLLEGE OF SOOCHOW UNIVERSITY,SUZHOU 215123,CHINA;2.SHANG-HAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHAN   

  • Online:2010-02-25 Published:2010-02-28

被引次数

43

摘要： 目的建立一种基于TaqMan探针技术的快速、准确且经济的实时荧光PCR方法,用于检测X染色体上的单核苷酸多态性(single nucleotide polymorphism,SNP)。方法选择X染色体上的13个SNP位点(X-SNP),针对每个位点分别设计1对PCR引物和TaqMan探针,进行实时荧光PCR扩增,对X-SNP位点进行分型。结果13个位点均符合Hardy-Weinberg平衡;多态信息含量分布为0.3497～0.3750,杂合度为0.4537～0.5021。建立的方法能够用于X-SNP位点的基因分型,检测结果与DNA测序结果完全一致。结论基于TaqMan探针技术的等位基因特异的实时荧光PCR方法灵敏、简单、快速,可实现对X-SNP位点的分型检测;所选择的13个X-SNP位点具有高信息量,在法医遗传学中具有潜在的应用价值。

关键词: 法医遗传学, 多态性, 单核苷酸, X染色体, TaqMan探针

Abstract: Objective To develop a rapid,accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism(X-SNP).Methods Taq-Man probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP.Then,the X-SNP were genotyped after the amplification by real time fluorescence PCR.Results All the loci follow the Hardy-Weinberg equilibrium.The polymorphic information content for 13 distinct loci varied between 0.349 7 and 0.375 0 while the heterozygosity ranged from 0.453 7 to 0.502 1.A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.Conclusion The allele specific real time fluores-cence PCR based on TaqMan probe is a sensitive,simple technology and suitable for rapid analysis of X-SNP.All the loci show highly polymorphic and may be potential in forensic genetics.

Key words: forensic genetics, polymorphism, single nucleotide, X chromosome, TaqMan probe