法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (2): 104-107.

• 论文 • 上一篇    下一篇

郑州地区食尸性苍蝇及幼虫mtDNA中COⅡ基因序列分析

赵祖亮;闫红涛;李世才;刘光旭;沈欣堂;   

  1. 郑州大学法医学教研室;
  • 发布日期:2010-04-25 出版日期:2010-04-28

Sequencing of Mitochondrial DNA COⅡ Gene in Carrion-Breeding Flies and Larvae in Zhengzhou

ZHAO ZU-LIANG,YAN HONG-TAO,LI SHI-CAI,LIU GUANG-XU,SHEN XIN-TANG (DEPARTMENT OF FORENSIC MEDICINE,ZHENGZHOU UNIVERSITY,ZHENGZHOU 450001,CHINA)   

  • Online:2010-04-25 Published:2010-04-28

摘要： 目的用mtDNA中细胞色素氧化酶辅酶Ⅱ(COⅡ)基因序列鉴定法医学中常见食尸性苍蝇及其幼虫的种类。方法收集郑州地区大白鼠尸体上的苍蝇及其幼虫,提取DNA,PCR扩增COⅡ序列,测序,用Clustalx和MEGA4.0软件对基因序列进行比对分析及构建系统进化树。结果成虫与幼虫基因差异不明显,COⅡ基因序列可以对棕尾别麻蝇、巨尾阿丽蝇和亮绿蝇进行鉴定,铜绿蝇与丝光绿蝇进化距离较近,COⅡ序列不能将他们区分开,同时还发现巨尾阿丽蝇和亮绿蝇存在种群单核苷酸多态性。结论mtDNA中COⅡ序列能有效鉴定郑州地区部分常见食尸性苍蝇的种类,方法简便、准确。

关键词: 法医遗传学, 昆虫学, 蝇科, DNA, 线粒体, 细胞色素氧化酶辅酶Ⅱ

Abstract: Objective Using COⅡ sequences to identify common species of carrion-breeding flies and larvae. Methods flies and larvae were collected on the corpses of rats in Zhengzhou district,DNA was extracted,COⅡ sequences were amplified and sequenced. Clustalx and MEGA 4.0 software were used to analyze the gene sequences and to construct the phylogenetic trees. Results There was no significant gene difference between adults and larvae. COⅡ gene sequences could be used to identify Boettcherisca peregrina,Aldrichina grahami and Lucilia illustris but they could not distinguish Lucilia cuprina from the Lucilia sericata because of their close evolutionary distance and single nucleotide polymorphisms in aldrichina grahami and Lucilia illustris populations were found. Conclusion COⅡ sequence of mtDNA in Zhengzhou district can be used effectively to identify some common species of carrion-breeding fly. The method is simple and accurate.

Key words: forensic genetics, entomology, muscidae, DNA, mitochondrial, COⅡ