法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (3): 175-178.DOI: 10.3969/j.issn.1004-5619.2012.03.004

• 论著 • 上一篇    下一篇

前列腺素D2、羧肽酶A3和血小板活化因子在过敏性休克豚鼠体内含量变化

杨  凯1，郭相杰1，闫学斌1，2，高彩荣1   

  1. （1. 山西医科大学法医学院法医病理学教研室，山西 太原 030001； 2. 清徐县公安局技术科，山西 太原 030012）
  • 发布日期:2012-06-25 出版日期:2012-06-28
  • 通讯作者: 高彩荣，女，博士，教授，博士研究生导师，主要从事法医病理学教学、研究和检案工作；E-mail：gaocairong5175@163.com
  • 作者简介:杨凯（1985—），男，山西临汾人，硕士研究生，主要从事法医病理学研究；E-mail：mryangk@163.com
  • 基金资助:

    山西省回国留学人员科研资助项目（2009-51）；山西省国际科技合作项目（2010081063）

Changes of Prostaglandin D2，Carboxypeptidase A3 and Platelet Activating Factor in Guinea Pig in Anaphylactic Shock

YANG KAI1, GUO XIANG-JIE1, YAN XUE-BIN1,2, GAO CAI-RONG1   

  1. （1. Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Technology Department of Qingxu Public Security Bureau, Taiyuan 030012, China）
  • Online:2012-06-25 Published:2012-06-28

摘要： 目的 观察白三烯E4（leukotriene E4，LTE4）、前列腺素D2（prostaglandin D2，PGD2）、羧肽酶A3（carboxypeptidase A3，CPA3）和血小板活化因子（platelet activating factor，PAF）在过敏性休克死亡豚鼠体内的变化规律。 方法 建立豚鼠过敏性休克的动物模型。ELISA法检测过敏性休克死亡豚鼠尿液中LTE4、血浆中PGD2和CPA3、脑组织中PAF的含量，与对照组比较筛选出有差别的指标。观察比较PGD2、CPA3和PAF在豚鼠过敏性休克0、12、24 h的变化规律，以及血小板活化因子乙酰水解酶（platelet activating factor acetylhydrolase，PAF-AH）对豚鼠脑组织中PAF的影响。 结果 LTE4的含量在两组间差异无统计学意义，过敏性休克死亡0 h豚鼠体内PGD2、CPA3、PAF含量较对照组明显升高；PGD2和CPA3含量在死后12 h下降至对照组水平，PAF含量死后12、24 h仍明显高于对照组；PAF-AH预处理能明显降低PAF含量。 结论 尿液中LTE4不适宜作为过敏性休克死亡的诊断指标；血浆中CPA3、PGD2及脑组织中PAF在死后一定时间内可作为过敏性休克死亡的法医学诊断指标；PAF-AH有望在临床上用于过敏性休克的治疗。

关键词: 法医病理学, 过敏反应, 白三烯E4, 前列腺素D2, 羧肽酶A3, 血小板活化因子, 豚鼠

Abstract: Objective To detect the changes of leukotriene E4（LTE4）, prostaglandin D2（PGD2）, carboxypeptidase A3（CPA3） and platelet activating factor（PAF） in guinea pigs died from anaphylactic shock. Methods Guinea pigs were used for establishing anaphylactic shock models. The levels of LTE4, PGD2 and CPA3, and PAF were detected in urine, plasma, and brain tissues with ELISA kit, respectively. The significant biomarkers were selected comparing with control group. The changes of PGD2, CPA3 and PAF in the guinea pigs at time zero, 12 and 24 hours after death were observed and compared respectively. The effect of platelet activating factor acetylhydrolase（PAF-AH） to PAF in guinea pig brain was examined and compared. Results There were no statistically differences of LTE4 levels in urine observed between experimental group and control group. The levels of CPA3, PGD2 and PAF in the experimental group were significantly higher than that in the control group at 0 h. The levels of PAF at 12 and 24 hours after anaphylactic shock were significantly higher than that in the control group. The levels of PAF decreased significantly after pretreatment with PAF-AH. Conclusion LTE4 in urine cannot be selected as a biomarker to determine the anaphylactic shock. PGD2 and CPA3 in plasma, and PAF in brain tissue may be used as biomarkers to determine the anaphylactic shock. PAF-AH may be potentially useful for clinical treatment of anaphylactic shock.

Key words: forensic pathology, anaphylaxis, leukotriene E4, prostaglandin D2, carboxypeptidase A3, platelet activating factor, guinea pigs

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