法医学杂志 ›› 2014, Vol. 30 ›› Issue (2): 81-84.DOI: 10.3969/j.issn.1004-5619.2014.02.001

• 论著 •    下一篇

溺死及死后入水兔肺组织中硅藻分布

李向阳1,2,赵  建3,刘  超3,胡孙林3,张又川4,温锦锋3,成建定   

  1. (1. 中山大学中山医学院法医学系,广东 广州 510080; 2. 广东省广州市公安局南沙区分局,广东 广州 511457; 3. 广州市刑事科学技术研究所 法医病理学公安部重点实验室,广东 广州 510030; 4. 厦门市铁路公安处,福建 厦门 361000)
  • 发布日期:2014-04-25 出版日期:2014-04-28
  • 通讯作者: 通信作者:刘超,男,教授,主任法医师,主要从事个体识别及死因鉴定研究;E-mail:liuchaogaj@21cn.com 通信作者:成建定,男,教授,主要从事法医病理学研究;E-mail:Chengjd@mail.sysu.edu.cn
  • 作者简介:李向阳(1971—),男,北京人,硕士研究生,副主任法医师,从事法医病理学应用研究;E-mail:lxy020108@163.com

Diatoms in Drowned and Postmortem Immersed Rabbits’ Lungs

LI XIANG-YANG1,2, ZHAO JIAN3, LIU CHAO3, HU SUN-LIN3, ZHANG YOU-CHUAN4, WEN JIN-FENG3, CHENG JIAN-DING1   

  1. (1. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China; 2. Nansha Branch of Guangzhou Public Security Bureau, Guangzhou 511457, China; 3. Key Laboratory of the Ministry of Public Security of Forensic Pathology, Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China; 4. Xiamen Railway Public Security Department, Xiamen 361000, China)
  • Online:2014-04-25 Published:2014-04-28
  • Supported by:

    公安部重点研究计划项目(2010ZDYJGDST017)

摘要: 目的 研究溺死及死后入水兔肺组织中硅藻含量、大小和种类的差异。 方法 将62只新西兰大白兔随机分为生前入水组(n=30)、死后入水组(n=30)和陆地死亡组(n=2),采用微波消解-扫描电镜法对各肺叶中的硅藻进行定性和定量分析。 结果 生前入水组各肺叶中均能检出硅藻,以小环藻、直链藻为主;死后入水组肺叶中以小环藻为主,某些肺叶中可无硅藻检出。左肺尖叶、右肺中间叶、右肺心叶的硅藻检出率在两组间差异有统计学意义(P<0.05)。 结论 采用微波消解-扫描电镜法分析生前溺水与死后入水兔肺组织内硅藻含量、大小和种类的分布,为法医学硅藻检验理论提供了一定的参考。

关键词: 法医病理学, 溺水, 硅藻类,

Abstract:  Objective To investigate the numbers, sizes and types distribution of diatoms in drowned and postmortem immersed rabbits’ lungs. Methods Sixty-two rabbits were randomly divided into drowning group (n=30), postmortem immersion group (n=30) and land death group (n=2), and the diatoms in each lung lobe were analyzed quantitatively and qualitatively by microwave digestion and scanning electron microscopy. Results In the drowning group, the diatoms were detected in each lung lobe with Cyclotella and Melosira in the majority. In the postmortem immersion group, Cyclotella was in the majority. And the diatoms weren’t detected in some lung lobes in postmortem immersion. There were significant differences in the detection rates of upper lobe of left lung, middle lobe and cardiac lobe of right lung in two groups (P<0.05). Conclusion Based on the microwave digestion and scanning electron microscopy, the numbers, sizes and types distribution of diatoms in drowned and postmortem immersed rabbits’ lungs can be analyzed and used as references for testing theory.

Key words: forensic pathology, drowning, diatoms, rabbits

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