法医学杂志

法医学杂志 ›› 1996, Vol. 12 ›› Issue (2): 0-0.

• 论文 •    

直接同步扩增5种基因位点

李小明，李生斌   

  1. 西安医科大学
  • 发布日期:1996-04-25 出版日期:1996-04-28

Simultaneously Typing of five Genetic Loci

LI XIAOMING; LI SHENGBIN (XI'AN MEDICAL UNIVERSITY;SHAANXI 710061)   

  • Online:1996-04-25 Published:1996-04-28

被引次数

6

摘要： 来用直接同步ＰＣＲ扩增５种基因位点及反向打点杂交技术，使扩增产物ＤＮＡ与膜探针条杂交。再通过酶联底物显色反应对ＬＤＬＲ、ＧＹＰＡ、Ｄ７Ｓ８、Ｇｃ和ＨＢＧＧ进行联合分型。该方法特别适用于微量、陈旧或降解的ＤＮＡ样本，其操作简便、迅速，应用在亲子鉴定案例，亲子关系概率高达９９．７５％。该技术在法医学、遗传学及临床诊断中具有重要价值，并将改变ＤＮＡ分型技术难于标准化的局面．

关键词: DNA分型, PCR, 亲子鉴定

Abstract: Five genetic Loci LDLR. GYPA. D7S8. GC and HBGG were simultaneously analyzed by PCR and the reverse dot blot typing technology. being quick and easy, this approach was suitable for the test of degraded DNA and could be used widely in forensic, genetic research and clinical diagnosis.

Key words: PCR, DNA typing, paternity testing, allele