法医学杂志

法医学杂志 ›› 1998, Vol. 14 ›› Issue (4): 193-194+.

• 论文 •    下一篇

上海地区D1S80位点基因频率分布及其在亲子鉴定中的应用

阙庭志,林源,李莉   

  1. 司法部司法鉴定科学技术研究所!上海,200063,司法部司法鉴定科学技术研究所!上海,200063,司法部司法鉴定科学技术研究所!上海,200063
  • 发布日期:1998-08-25 出版日期:1998-08-28

PCR-based analysis of the D1S80 locus and its application in paternity test

QUE TINGZHI; LIN YUAN; LI LI(INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, PRC, SHANGHAI 200063)   

  • Online:1998-08-25 Published:1998-08-28

被引次数

19

摘要： 目的：将D1S80位点的DNA多态性分析应用于亲子鉴定。方法；PCR、聚丙烯酰胺凝胶电泳及溴已锭染色。结果：获得D1S80位点的DNA多态性分布数据。结论：D1S80位点的PCR检测方法可成功地用于亲权纠纷案的鉴定。

关键词: D1S80, 上海地区频率分布, PCR, 亲子鉴定

Abstract: Allelic data for the DIS80 locus was obtained by usingthe PCR and subsequent analysis with high - resolution horizontal PAGE technique. In a sample of 184 unrelated Chinese, the D1S80 locus had 75 phenotypes and 20 allelic.The distribution of genotype was in agreement with expectedvalues according to the Hardy - Weinberg equilibrium. Analysis of the D1S80 locus by the PCR has been successfully applied in 150 cases of questioned paternity.

Key words: chelex, D1S80 locus, DNA, PCR, paternity test